Hello,

I would like to use the Variance Stabilizing Transformation (VST) function on a dataset that has only one condition. In order to generate a DESeqDataSet (dds) for the dataset I have to input at least two conditions. Would it work to artificially assign two conditions for the dataset and then perform the VST blinded so it does not account for the assigned conditions?

Thanks for your help.

You can use design= ~1