Hi There,
I had a question regarding using msstats from an output generated by
MaxQuant output on TMT results. The issue is when I use proteingroup
command on the output that I got from protein summarization command on
msstat.
There are 2 type files hat are very similar, one file gives me the
correct proteingroup output whereas the other one looks like all SE,DF
pvalue,adj.pvalue columns are missing their values. I have tried it
many times over and over and the step before which is protein
summarization looks normal in both, but one file does not give the
correct protein Group.
I can send you the file that works ( GroupComparision-I) and the
other file that did not work(GroupComparision-M)
I appreciate any help. I can also send you all of my R commands
Thanks.
Regards,
Yasi Jami
First, I'm wondering whether you use MSstatsTMT package, instead of MSstats. MSstats supports DDA, DIA, SRM. MSstatsTMT supports TMT. If you use MSstats, please try MSstatsTMT instead.
And There are several points that are not clear to us. It will be better to share your R scripts with us in order to understand what steps you are not clear and which outputs you claim.
Hope that it can be helpful.
- Meena