Question: covert from RGList to uRNAList
0
gravatar for ahadli.farid
10 months ago by
ahadli.farid0 wrote:

Hello all,

I was trying to use readMicroRnaAFE() function of AgiMicroRna packge. However, one of the required columns for readMicroRnaAFE() was missing from the raw data. Afterwards I used read.maimage() function of limma package to read the file. The problem is that read.maimage() outputs an RGList object but I need to convert it to uRNAList object in order to continue downstream analyses with AgiMicroRna package. I would appreciate any help.

Regards,

Farid Ahadli.

agimicrorna agilent • 174 views
ADD COMMENTlink modified 10 months ago by Gordon Smyth38k • written 10 months ago by ahadli.farid0
Answer: covert from RGList to uRNAList
0
gravatar for Gordon Smyth
10 months ago by
Gordon Smyth38k
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Gordon Smyth38k wrote:

The short answer is that you can't convert an RGList into a uRNAList.

You've already confirmed that your raw data doesn't contain all the information needed to make a uRNAList. Going through the intermediary of an RGList can't change that.

On the other hand, there are probably plenty of ways to analyse the data without creating a uRNAList.

 

 

ADD COMMENTlink written 10 months ago by Gordon Smyth38k

Thank you Gordon Smyth! Could you please name few of the options, other than limma itseff? The reason why we opted for AgiMicroRna was its convenience. 

ADD REPLYlink written 10 months ago by ahadli.farid0
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