Hello all,

I was trying to use readMicroRnaAFE() function of AgiMicroRna packge. However, one of the required columns for readMicroRnaAFE() was missing from the raw data. Afterwards I used read.maimage() function of limma package to read the file. The problem is that read.maimage() outputs an RGList object but I need to convert it to uRNAList object in order to continue downstream analyses with AgiMicroRna package. I would appreciate any help.

Regards,

Farid Ahadli.

The short answer is that you can't convert an RGList into a uRNAList.

You've already confirmed that your raw data doesn't contain all the information needed to make a uRNAList. Going through the intermediary of an RGList can't change that.

On the other hand, there are probably plenty of ways to analyse the data without creating a uRNAList.