affy: How to back transform the matrix to a vector? (low-level imaging)
1
0
Entering edit mode
Simon Lin ▴ 210
@simon-lin-461
Last seen 9.7 years ago
I am trying to transform the vector (pm and mm) extracted from @exprs to a matrix (2-D layout), so an image can be plotted. It works out. Now, when I back-transform the image matrix to the vector, there are some trouble. See the code below. Seems that the matrix has to be transposed somehow. Any ideas? Thanks! -Simon library (affy) data (affybatch.example) # has 3 chips x<- affybatch.example m.vector <- log2( x at exprs[, 2]) # take the second array x.pos <- (1:nrow(x)) - (1 + getOption("BioC")$affy$xy.offset) y.pos <- (1:ncol(x)) - (1 + getOption("BioC")$affy$xy.offset) # from vector to matrix m <- as.matrix(rev(as.data.frame(matrix(m.vector, nrow = length(x.pos), ncol = length(y.pos))))) # ???????????????? # from matrix to vector m0.vector<- as.vector (m) # this does not work, need some massage of m # see if it works sum (m.vector!= m0.vector)
• 737 views
ADD COMMENT
0
Entering edit mode
@kasper-daniel-hansen-2979
Last seen 10 months ago
United States
On Jan 31, 2006, at 4:34 PM, Simon Lin wrote: > I am trying to transform the vector (pm and mm) extracted from > @exprs to a > matrix (2-D layout), so an image can be plotted. It works out. Yes. I assume the code given below has been snapped from getMethod("image", "AffyBatch")\ (Why do you want to generate that code anyway, when suitable image functions exists?) > > > Now, when I back-transform the image matrix to the vector, there > are some > trouble. See the code below. > > > > Seems that the matrix has to be transposed somehow. Any ideas? > Thanks! -Simon > > > > library (affy) > > data (affybatch.example) # has 3 chips > > x<- affybatch.example > > m.vector <- log2( x at exprs[, 2]) # take the second array > > > > x.pos <- (1:nrow(x)) - (1 + getOption("BioC")$affy$xy.offset) > > y.pos <- (1:ncol(x)) - (1 + getOption("BioC")$affy$xy.offset) > > # from vector to matrix > > m <- as.matrix(rev(as.data.frame(matrix(m.vector, nrow = length > (x.pos), > > ncol = length(y.pos))))) If you debug this line, you will understand why a simple as.matrix does not work. Basically, first a matrix is created. It is then converted to a data.frame so that rev() works on columns. Look: > matrix(1:9, nrow = 3) [,1] [,2] [,3] [1,] 1 4 7 [2,] 2 5 8 [3,] 3 6 9 > as.data.frame(matrix(1:9, nrow = 3)) V1 V2 V3 1 1 4 7 2 2 5 8 3 3 6 9 > rev(as.data.frame(matrix(1:9, nrow = 3))) V3 V2 V1 1 7 4 1 2 8 5 2 3 9 6 3 > as.matrix(rev(as.data.frame(matrix(1:9, nrow = 3)))) V3 V2 V1 1 7 4 1 2 8 5 2 3 9 6 3 > as.vector(as.matrix(rev(as.data.frame(matrix(1:9, nrow = 3))))) [1] 7 8 9 4 5 6 1 2 3 Ok? /Kasper > > > # ???????????????? > > # from matrix to vector > > m0.vector<- as.vector (m) # this does not work, need some > massage of m > > # see if it works > > sum (m.vector!= m0.vector) > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor
ADD COMMENT

Login before adding your answer.

Traffic: 503 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6