Hi

I am using Deseq2 for Differential gene expression analysis. I have two controls ( but they are not technical replicates) and one patient.

I designed the experiment with two controls together. I was wondering what Deseq2 does when two controls are taken together. Does it takes an average of the two controls.

Many Thanks

Tanya Singh

I’m not sure I understand exactly but if you have control 1 and control 2 vs case, yes the LFC will be roughly case over the average of the controls.