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Mr.RB
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20
@mrrb-15633
Last seen 5.7 years ago
I found predORF
from systemPipeR
to predict the longest non-overlapping reading frames in a genome, however these are the longest ORFs per strand. I want to have the longest ORF considering both strands, for example:
AAAA**ATG**XXXXXX**TGA**AAAA CDS = XXXXXX = 6 nt <- pick this one
AAAATAGX**AGT**XXACT**GTA**A CDS = XXACT = 5 nt
Is there any package in R that can do this, I suppose it isn't that easy to code this myself since it requires quite some comparisons when both strands need to be considered.