Dear all,

I have .fcs files and aim to perform cell cycle analysis to determine the amount of apoptotic cells. I tried with FlowPloidy and get the peak for G1. However, I don't know how to proceed. I aim to determine G2 and the amount of cells in SubG1, G1, S and G2. I might have to extract the fitting paramters from my control sample and apply it to the treated samples. But I do not know. Please suggest how to analyze cell cycle in R.

Here is an example of what I aim to do

http://www.icms.qmul.ac.uk/flowcytometry/uses/apoptosis/dnafragmentation/

http://www.icms.qmul.ac.uk/flowcytometry/uses/necroptosis/necroptoticarrest.gif

Kind regards, Nina