I am using the ddCt package for evaluation of qPCR data and it works fine with the default settings. Is it possible to use gene specific efficiencies? In the vingnette it is mentioned that it is possible to use them but I have no clue where to change the default parameter thatsets equal gene efficiencies for all genes. Where can I change paramerters?

Did you read (one of) the vignettes. Seems like it's covered in sufficient detail.

Hi bettenbrock, as suggested by James, please have a look at the vignette and check whether your problem is solved.

If not, as the package author, I will be happy to help further. Please contact me with a minimal example in that case.

Best regards, David