Hello,

I am new to RNA-seq. I have output from tximport and I want to use DESeq2. I have used five replicates for each of two treatments and I want to check the difference. I am not sure which design to choose for DSeq2.

Thank you!

All Bioconductor packages have extensive documentation and vignettes, which you should read over before posting to the support site. You can access them from R with vignette(“DESeq2”) or you can read from the website.