Hi, I have a gene expression data in cpm not raw count and I want to do DE analysis using edgeR. In this case, is it okay to follow the overall workflow shown in the edgeR user guide because it's not raw counts in integer...? I'm wondering if it's okay to do "calcNormFactors(y)" where y is the DGEList of the data in cpm.
x is my data that contains gene expression values in cpm.
x <- read.table (file~) : this is cpm values of each gene in each samples.`
y <- DGEList(x)
y <- calcNormFactors(y)
thanks in advance.