Reading flag data from Agilent files
0
0
Entering edit mode
@giovanni-coppola-893
Last seen 9.6 years ago
Hello Frederic, you might be interested in this thread: http://article.gmane.org/gmane.science.biology.informatics.conductor/ 1822/ Best Giovanni On May 19, 2006, at 8:27 AM, Frederic Capel wrote: > Cher Monsieur, je viens demander un peu d'aide sur l'utilisation du > package Limma: j'esp?re que vous allez pouvoir m'aider! > je suis tomb? sur une question ancienne relative au flag de spots > lorsque > Limma est utilis? pour traiter des data issues de F extraction > d'agilent, > j'ai un souci comparable ? celui que vous aviez: je n'arrive pas ? > g?rer > les flags: pouvez vous me renseigner sur la commande ? introduire ? > dans > mon objet Rg je n'ai pas de weight, ce qui ?tait le cas lorsque je > travaillais avec Genepix et des .gpr > Merci par avance > > mon script pr?liminaire sans fonction pour les flags car rien ne > marche: > targets<- readTargets("target1.txt",row.names="Slide number") > RG <-read.maimages(targets$FileName,source="agilent") > #Read 251239140435.txt > #Read 251239140486.txt > #Read 251239140436.txt > #Read 251239140487.txt > #Read 251239140465.txt > #Read 251239140534.txt > #Read 251239140537.txt > #Read 251239140460.txt > #Read 251239140539.txt > #Read 251239140484.txt > #Read 251239143844.txt > #Read 251239140540.txt > > names(RG) > #[1] "G" "Gb" "R" "Rb" "targets" "genes" > "source" > names(RG$genes) > #[1] "Row" "Col" "ProbeUID" "ControlType" > #[5] "ProbeName" "GeneName" "SystematicName" "Description" > > > > > Votre message ?tait: > Hi list, > > does somebody here knows if there's a function in the > Bioconductor packages for reading flags data (feature > saturation, non uniformity, etc...) or other kind of > data from Agilent files (from Feature Extraction > software). I used read.Agilent(marray) but it doesn't > read other data that Fg and Bg intensities, wrong ? Or > is there something to do with the '... further > arguments to scan(base)' ? > > > > > > Frederic Capel, Ph D > PostDoc > INSERM U586, Obesity Research Unit > IFR31, Institut Louis Bugnard, BP 84225 > 31432 TOULOUSE Cedex 4 > Frederic.Capel at toulouse.inserm.fr > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor
limma limma • 655 views
ADD COMMENT

Login before adding your answer.

Traffic: 692 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6