Entering edit mode
Hi,
I was just wondering if it okay to use mixed model in DE analysis with deseq2. If yes, in what circumstances. For example, I have multiple tissue samples collected from the same individual where my variable of interest is a disease. I have multiple categorical variable associated with the samples such as age, sex, RIN, PMI. Which model will be more appropriate,
formula = ~ age + sex + RIN + PMI + tissue + disease
or
formula = ~ (1| individual) + RIN + PMI + disease
Thanks in advance.
Nurun
Hi, thanks for clarifying.