Question

Differential gene expression using DESeq2 on Ribo-Seq data

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DataFanatic ▴ 10

@datafanatic-12212

Last seen 3.4 years ago

Is it possible to normalize and perform differential gene expression using DESeq2 on Ribo-Seq data? Thanks!

DESeq2 Ribo-Seq differential gene expression • 979 views

ADD COMMENT • link 3.7 years ago DataFanatic ▴ 10

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site:support.bioconductor.org Ribo-Seq DESeq2 I only found one post https://support.bioconductor.org/p/111743/

In my case, I only have Ribo-Seq data control and treatment and my basic question is can I treat these data as RNA-Seq data for the purpose of differential translation?

I have used other packages to do Ribo-Seq specific QC.

ADD REPLY • link 3.7 years ago DataFanatic ▴ 10

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Yes you can do enriched vs control.

ADD REPLY • link 3.7 years ago Michael Love 41k

score 1 · Answer 1 · 2020-07-30

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Michael Love 41k

@mikelove

Last seen 1 day ago

United States

Can you poke around the support site (try google with “site:”). There are a number of posts on this.

ADD COMMENT • link 3.7 years ago Michael Love 41k