Missing Point in PCA Analysis
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pthom010 • 0
@pthom010-23694
Last seen 2.3 years ago
United States

I conducted my DESeq analysis on 30 samples split between 2 genotypes, 5 time points and 3 replicates (30 samples total - 15 per genotype). I tried to make my PCA plot but I seem to be missing 2 points ( 1 from each genotype). when I ask R how many samples it sees in the dds object, it indicates 30 so I don't get why I am missing points? I've attached an image below.

https://ibb.co/s1FXnZX

deseq2 • 557 views
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@mikelove
Last seen 3 days ago
United States

They could be overlapping. Try returnData=TRUE.

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