Hi,

I want to use deseq2 for RNAseq analysis.

I have excel file of RNA count data with one column is geneid and others are different patients information.

It looks like

          patient1 patient2 patient3 patient1beforedisease patient2beforedisease patient3beforedisease

Gene1 RNAcount RNAcount RNAcount RNAcount RNAcount RNAcount Gene2 RNAcount RNAcount RNAcount RNAcount RNAcount RNAcount Gene3 RNAcount RNAcount RNAcount RNAcount RNAcount RNAcount . . .

I am wondering how to analysis data for that I want to find the genes that changed before and after disease. How can I transform excel data format into the inputting data for the software, that is to say, what kinds of format the software recognize.

Thanks!

DESeq2 has extensive documentation that will help you work with the functions, including data import. The support site is here to help answer software questions but not to act as a replacement for reading the documentation. If you’ve never worked with R before you may want to recruit someone with R experience to help you get through eg importing tabular data into R.