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@jmungalberkeleyedu-1797
Last seen 10.6 years ago
Hello, I am an undergraduate doing summer research at Stanford University. I've downloaded a few GSP files from GEO and converted them to the class exprSet. Typically with an object of class exprSet you can simply type > annotation(exprSet.object) and it R will give you the annotation key for the corresponding LocusLink ID. However, with the data sets I have, there is no annotation. Does anyone know an easy way of finding the LocusLink ID's for such a data set? Thanks very much for any help. -Justin Mungal
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Seth Falcon ★ 7.4k
@seth-falcon-992
Last seen 10.6 years ago
Hi Justin, jmungal at berkeley.edu writes: > Hello, I am an undergraduate doing summer research at Stanford University. > I've downloaded a few GSP files from GEO and converted them to the class > exprSet. Typically with an object of class exprSet you can simply type > >> annotation(exprSet.object) > > and it R will give you the annotation key for the corresponding LocusLink > ID. I'm not so sure about that. The annotation() method for the exprSet class is AFAIK a simple accessor for the annotation slot of said class. This slot is intended to hold a string naming the annotation package appropriate for the data contained in the exprSet. > However, with the data sets I have, there is no annotation. Does > anyone know an easy way of finding the LocusLink ID's for such a > data set? The probe ID ==> EntrezGene ID mapping is chip dependent. For many Affymetrix chips, there is a BioC annotationd data package available. That is not the case for two-color chips. + seth
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Seth Falcon wrote: > Hi Justin, > > jmungal at berkeley.edu writes: > >>Hello, I am an undergraduate doing summer research at Stanford University. >> I've downloaded a few GSP files from GEO and converted them to the class >>exprSet. Typically with an object of class exprSet you can simply type >> >> >>>annotation(exprSet.object) >> >>and it R will give you the annotation key for the corresponding LocusLink >>ID. > > > I'm not so sure about that. The annotation() method for the exprSet > class is AFAIK a simple accessor for the annotation slot of said > class. This slot is intended to hold a string naming the annotation > package appropriate for the data contained in the exprSet. > > >>However, with the data sets I have, there is no annotation. Does >>anyone know an easy way of finding the LocusLink ID's for such a >>data set? Justin, I don't think there is such a thing as a GSP from GEO. If you clarify what you mean by GSP, I might be able to help you get the associated platform (GPL) information from GEO using GEOquery. You can then use that information for your annotation (which may or may not satisfy your needs, depending on what is available in the GPL). Sean
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