Hello, I am an undergraduate doing summer research at Stanford
University.
I've downloaded a few GSP files from GEO and converted them to the
class
exprSet. Typically with an object of class exprSet you can simply
type
> annotation(exprSet.object)
and it R will give you the annotation key for the corresponding
LocusLink
ID. However, with the data sets I have, there is no annotation. Does
anyone know an easy way of finding the LocusLink ID's for such a data
set?
Thanks very much for any help.
-Justin Mungal
Hi Justin,
jmungal at berkeley.edu writes:
> Hello, I am an undergraduate doing summer research at Stanford
University.
> I've downloaded a few GSP files from GEO and converted them to the
class
> exprSet. Typically with an object of class exprSet you can simply
type
>
>> annotation(exprSet.object)
>
> and it R will give you the annotation key for the corresponding
LocusLink
> ID.
I'm not so sure about that. The annotation() method for the exprSet
class is AFAIK a simple accessor for the annotation slot of said
class. This slot is intended to hold a string naming the annotation
package appropriate for the data contained in the exprSet.
> However, with the data sets I have, there is no annotation. Does
> anyone know an easy way of finding the LocusLink ID's for such a
> data set?
The probe ID ==> EntrezGene ID mapping is chip dependent. For
many Affymetrix chips, there is a BioC annotationd data package
available. That is not the case for two-color chips.
+ seth
Seth Falcon wrote:
> Hi Justin,
>
> jmungal at berkeley.edu writes:
>
>>Hello, I am an undergraduate doing summer research at Stanford
University.
>> I've downloaded a few GSP files from GEO and converted them to the
class
>>exprSet. Typically with an object of class exprSet you can simply
type
>>
>>
>>>annotation(exprSet.object)
>>
>>and it R will give you the annotation key for the corresponding
LocusLink
>>ID.
>
>
> I'm not so sure about that. The annotation() method for the exprSet
> class is AFAIK a simple accessor for the annotation slot of said
> class. This slot is intended to hold a string naming the annotation
> package appropriate for the data contained in the exprSet.
>
>
>>However, with the data sets I have, there is no annotation. Does
>>anyone know an easy way of finding the LocusLink ID's for such a
>>data set?
Justin,
I don't think there is such a thing as a GSP from GEO. If you clarify
what you mean by GSP, I might be able to help you get the associated
platform (GPL) information from GEO using GEOquery. You can then use
that information for your annotation (which may or may not satisfy
your
needs, depending on what is available in the GPL).
Sean