gcrma and combineAffyBatch
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Lo, Ken ▴ 50
@lo-ken-1904
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Lo, Ken ▴ 50
@lo-ken-1904
Last seen 9.6 years ago
Hi all, As I am a newbie to the bioconductor world, I would like to ask a question that Zhijin Wu and Vincent has touched upon. I would like to use combineAffyBatch to combine a set of U133A and U133Plus2 expression arrays. I have done that sucessfully with combineAffyBatch and ran RMA on the combined dataset sucessfully. However, when I tried running gcrma on the combined dataset, it throws an error: Adjusting for optical effect.....Done. Computing affinities[1] "Checking to see if your internet connection works..." [1] "Environment tempcdfcdf was not found in the Bioconductor repository." [1] "Checking to see if your internet connection works..." [1] "Environment tempcdfprobe was not found in the Bioconductor repository." Error in get(x, envir, mode, inherits) : variable "tempcdfprobe" was not found I tried applying the solution that was provided by Wu (in response to Vincent Detours) but failed as the dimensions of the affinity.info1=new("AffyBatch", exprs=exprs(affinity.info1),cdfname(affinity.info1), nrow=640, ncol=640) statement is not right for the U133A and U133Plus2 platform. Would anyone be able to guide me to do this? Best regards, Ken Lo ********************************************** Ken Lo, PhD Post-Doctorate Fellow Department of Cancer Genetics Center for Genetics and Pharmacology Roswell Park Cancer Institute Elm and Carlton Streets Buffalo, New York 14263 Telephone: 716-845-3941 Fax: 716-845-3940 E-mail: Ken.Lo at RoswellPark.org Web: www.RoswellPark.org <http: www.roswellpark.org=""/> Located in the Buffalo Life Science Complex on the Buffalo Niagara Medical Campus ********************************************** This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.
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