Hi all,
As I am a newbie to the bioconductor world, I would like to ask a
question that Zhijin Wu and Vincent has touched upon. I would like to
use combineAffyBatch to combine a set of U133A and U133Plus2
expression
arrays. I have done that sucessfully with combineAffyBatch and ran
RMA
on the combined dataset sucessfully. However, when I tried running
gcrma on the combined dataset, it throws an error:
Adjusting for optical effect.....Done.
Computing affinities[1] "Checking to see if your internet connection
works..."
[1] "Environment tempcdfcdf was not found in the Bioconductor
repository."
[1] "Checking to see if your internet connection works..."
[1] "Environment tempcdfprobe was not found in the Bioconductor
repository."
Error in get(x, envir, mode, inherits) : variable "tempcdfprobe" was
not
found
I tried applying the solution that was provided by Wu (in response to
Vincent Detours) but failed as the dimensions of the
affinity.info1=new("AffyBatch",
exprs=exprs(affinity.info1),cdfname(affinity.info1), nrow=640,
ncol=640)
statement is not right for the U133A and U133Plus2 platform. Would
anyone be able to guide me to do this?
Best regards,
Ken Lo
**********************************************
Ken Lo, PhD
Post-Doctorate Fellow
Department of Cancer Genetics
Center for Genetics and Pharmacology
Roswell Park Cancer Institute
Elm and Carlton Streets
Buffalo, New York 14263
Telephone: 716-845-3941
Fax: 716-845-3940
E-mail: Ken.Lo at RoswellPark.org
Web: www.RoswellPark.org <http: www.roswellpark.org=""/>
Located in the
Buffalo Life Science Complex
on the Buffalo Niagara Medical Campus
**********************************************
This email message may contain legally privileged and/or confidential
information. If you are not the intended recipient(s), or the
employee or agent responsible for the delivery of this message to the
intended recipient(s), you are hereby notified that any disclosure,
copying, distribution, or use of this email message is prohibited. If
you have received this message in error, please notify the sender
immediately by e-mail and delete this email message from your
computer. Thank you.