Human 50k Xba1 Affymetrix arrays cdf file
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@christophe-poulet-1926
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@james-w-macdonald-5106
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Hi Christophe, Christophe Poulet wrote: > > > > Hello, > > > I want to > study SNP arrays (Human 50k Xba1 Affymetrix) with Bioconductor, and I don?t > really know how to do, when I want to execute my cdf files from Gtype, R answer me that I > don?t have the 50k Xba1 package. I?ve search it on the bioconductor website, > but I didn't find it. > > > I think > that I must create this package with the cdf file here, but I > don?t know how to do, could you help me please? You need to install the oligo and makePlatformDesign packages. They are still developmental packages, so if you are using some version of R less than R-devel, you need to set your repository: repos <- "http://www.bioconductor.org/packages/2.0/bioc/" install.packages(c("oligo","makePlatformDesign"), repos=repos, dependencies=TRUE) On R-devel you can simply use biocLite(). You will also need the cdf file, the annotation file, and the probe sequence file, which can be downloaded from Affy's website. You can then make the PDEnv using makePDpackage(). You will then need to install it, instructions for doing so can be found in the R-FAQ (how can add-on packages be installed?) or in the OS-specific FAQs if you are on Windows or MacOS. HTH, Jim > > > > > > > Best > RegardsChristophe PouletLaboratoire de Cytog?n?tique, CHUUniversit? de Li?ge, Sart Tilmanchristophe.poulet at hotmail.com > _________________________________________________________________ > D?couvrez Windows Live Spaces et cr?ez votre site Web perso en quelques clics ! > > [[alternative HTML version deleted]] > > > > -------------------------------------------------------------------- ---- > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues.
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@christophe-poulet-1926
Last seen 10.3 years ago
Thanks for your answer, I've download and install the "oligo" and "makePDpackage", and the Zipfiles from Affymetrix with success, but R send another error, like this: > makePDpackage()affymetrix expressionError in basename(filein) : argument "designFile" is missing, with no defaultI tried to find what and where was the design file but I failed to find it.I forget to tell you that I am a Biologist who try to work on windows with RGui 2.4.0, it might be important, because I haven't got Computer Knowledge. You can find a word doc which contains what I have done on R. Could you tell me what to do, because I don't find this problem in the R FAQs for windows.Thanks for your time.Best regards.Christophe PouletLaboratoire de Cytog?n?tique, CHUUniversit? de Li?ge, Sart Tilmanchristophe.poulet at hotmail.com _________________________________________________________________ Lancez des recherches en toute s?curit? depuis n'importe quelle page Web. T?l?chargez GRATUITEMENT Windows Live Toolbar aujourd'hui !
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Hi Christophe, Christophe Poulet wrote: > Thanks for your answer, I've download and install the "oligo" and > "makePDpackage", and the Zipfiles from Affymetrix with success, but R > send another error, like this: > > > > > > >> makePDpackage()affymetrix expressionError in basename(filein) : >> argument You can't just call makePDpackage() with no arguments. You have to explicitly tell the function what files to use to make your package because it can't just figure it out on its own. If you type ?makePDpackage at an R prompt it will show you information about this function. However, simply making the package is only half the battle. You will also need to install it, which is not a simple task for someone without computer knowledge. You will probably need to find someone local who does have computer knowledge to help you get set up to install packages, and possibly install it for you. Information on how to do so can be found in the R FAQ for windows under section 4.1. Best, Jim > > "designFile" is missing, with no defaultI tried to find what and > where was the design file but I failed to find it.I forget to tell > you that I am a Biologist who try to work on windows with RGui 2.4.0, > it might be important, because I haven't got Computer Knowledge. You > can find a word doc which contains what I have done on R. > > Could you tell me what to do, because I don't find this problem in > the R FAQs for windows.Thanks for your time.Best regards.Christophe > PouletLaboratoire de Cytog?n?tique, CHUUniversit? de Li?ge, Sart > Tilmanchristophe.poulet at hotmail.com > _________________________________________________________________ > Lancez des recherches en toute s?curit? depuis n'importe quelle page > Web. T?l?chargez GRATUITEMENT Windows Live Toolbar aujourd'hui ! > http://toolbar.live.com -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues.
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Hi Christophe, Note that using makePDpackage under Windows, you must have the tools for building R Packages under your OS. http://www.murdoch-sutherland.com/Rtools/ The link above describe how to set up your system for this. To build the PDEnv for the XBA array you need the following files: Mapping50K_Xba240.CDF Mapping50K_Xba240_probe_fasta Mapping50K_Xba240_annot.csv Once you have these files in one directory, start R and change the working directory to the one where the files are (you can do this via menu/file/change directory or using the command setwd() ). Then you can use the command: makePDpackage("Mapping50K_Xba240.CDF", "Mapping50K_Xba240_probe_fasta", "Mapping50K_Xba240_annot.csv", type="SNP") to create the package... Now that the package is create you should build and install it. Let me know if you need any further help. b ps: The following page may be helpful: http://www.biostat.jhsph.edu/~kbroman/Rintro/Rwinpack.html On Nov 3, 2006, at 10:19 AM, Christophe Poulet wrote: > Thanks for your answer, > I've download and install the "oligo" and "makePDpackage", and the > Zipfiles from Affymetrix with success, but R send another error, > like this: > > makePDpackage() > affymetrix expression > Error in basename(filein) : argument "designFile" is missing, with > no default > > I tried to find what and where was the design file but I failed to > find it. > > I forget to tell you that I am a Biologist who try to work on > windows with RGui 2.4.0, it might be important, because I haven't > got Computer Knowledge. You can find a word doc which contains what > I have done on R. > > Could you tell me what to do, because I don't find this problem in > the R FAQs for windows. > Thanks for your time. > Best regards. > > Christophe Poulet > Laboratoire de Cytog?n?tique, CHU > Universit? de Li?ge, Sart Tilman > christophe.poulet at hotmail.com > > > Lancez des recherches en toute s?curit? depuis n'importe quelle > page Web. T?l?chargez GRATUITEMENT Windows Live Toolbar > aujourd'hui ! Essayez-le maintenant ! > <r.doc>
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