Hi Christophe,
Christophe Poulet wrote:
>
>
>
> Hello,
>
>
> I want to
> study SNP arrays (Human 50k Xba1 Affymetrix) with Bioconductor, and
I don?t
> really know how to do, when I want to execute my cdf files from
Gtype, R answer me that I
> don?t have the 50k Xba1 package. I?ve search it on the bioconductor
website,
> but I didn't find it.
>
>
> I think
> that I must create this package with the cdf file here, but I
> don?t know how to do, could you help me please?
You need to install the oligo and makePlatformDesign packages. They
are
still developmental packages, so if you are using some version of R
less
than R-devel, you need to set your repository:
repos <- "http://www.bioconductor.org/packages/2.0/bioc/"
install.packages(c("oligo","makePlatformDesign"), repos=repos,
dependencies=TRUE)
On R-devel you can simply use biocLite().
You will also need the cdf file, the annotation file, and the probe
sequence file, which can be downloaded from Affy's website. You can
then
make the PDEnv using makePDpackage(). You will then need to install
it,
instructions for doing so can be found in the R-FAQ (how can add-on
packages be installed?) or in the OS-specific FAQs if you are on
Windows
or MacOS.
HTH,
Jim
>
>
>
>
>
>
> Best
> RegardsChristophe PouletLaboratoire de Cytog?n?tique, CHUUniversit?
de Li?ge, Sart Tilmanchristophe.poulet at hotmail.com
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--
James W. MacDonald, M.S.
Biostatistician
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
7410 CCGC
Ann Arbor MI 48109
734-647-5623
**********************************************************
Electronic Mail is not secure, may not be read every day, and should
not be used for urgent or sensitive issues.
Thanks for your answer, I've download and install the "oligo" and
"makePDpackage", and the Zipfiles from Affymetrix with success, but R
send another error, like this:
> makePDpackage()affymetrix expressionError in basename(filein) :
argument
"designFile" is missing, with no defaultI tried to find what and where
was the design file but I failed to find it.I forget to tell you that
I am a Biologist who try to work on windows with RGui 2.4.0, it might
be important, because I haven't got Computer Knowledge. You can find a
word doc which contains what I have done on R.
Could you tell me what to do, because I don't find this problem in the
R FAQs for windows.Thanks for your time.Best regards.Christophe
PouletLaboratoire de Cytog?n?tique, CHUUniversit? de Li?ge, Sart
Tilmanchristophe.poulet at hotmail.com
_________________________________________________________________
Lancez des recherches en toute s?curit? depuis n'importe quelle page
Web. T?l?chargez GRATUITEMENT Windows Live Toolbar aujourd'hui !
Hi Christophe,
Christophe Poulet wrote:
> Thanks for your answer, I've download and install the "oligo" and
> "makePDpackage", and the Zipfiles from Affymetrix with success, but
R
> send another error, like this:
>
>
>
>
>
>
>> makePDpackage()affymetrix expressionError in basename(filein) :
>> argument
You can't just call makePDpackage() with no arguments. You have to
explicitly tell the function what files to use to make your package
because it can't just figure it out on its own.
If you type ?makePDpackage at an R prompt it will show you information
about this function.
However, simply making the package is only half the battle. You will
also need to install it, which is not a simple task for someone
without
computer knowledge. You will probably need to find someone local who
does have computer knowledge to help you get set up to install
packages,
and possibly install it for you. Information on how to do so can be
found in the R FAQ for windows under section 4.1.
Best,
Jim
>
> "designFile" is missing, with no defaultI tried to find what and
> where was the design file but I failed to find it.I forget to tell
> you that I am a Biologist who try to work on windows with RGui
2.4.0,
> it might be important, because I haven't got Computer Knowledge. You
> can find a word doc which contains what I have done on R.
>
> Could you tell me what to do, because I don't find this problem in
> the R FAQs for windows.Thanks for your time.Best regards.Christophe
> PouletLaboratoire de Cytog?n?tique, CHUUniversit? de Li?ge, Sart
> Tilmanchristophe.poulet at hotmail.com
> _________________________________________________________________
> Lancez des recherches en toute s?curit? depuis n'importe quelle page
> Web. T?l?chargez GRATUITEMENT Windows Live Toolbar aujourd'hui !
> http://toolbar.live.com
--
James W. MacDonald, M.S.
Biostatistician
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
7410 CCGC
Ann Arbor MI 48109
734-647-5623
**********************************************************
Electronic Mail is not secure, may not be read every day, and should
not be used for urgent or sensitive issues.
Hi Christophe,
Note that using makePDpackage under Windows, you must have the tools
for building R Packages under your OS.
http://www.murdoch-sutherland.com/Rtools/
The link above describe how to set up your system for this.
To build the PDEnv for the XBA array you need the following files:
Mapping50K_Xba240.CDF
Mapping50K_Xba240_probe_fasta
Mapping50K_Xba240_annot.csv
Once you have these files in one directory, start R and change the
working directory to the one where the files are (you can do this via
menu/file/change directory or using the command setwd() ).
Then you can use the command:
makePDpackage("Mapping50K_Xba240.CDF",
"Mapping50K_Xba240_probe_fasta", "Mapping50K_Xba240_annot.csv",
type="SNP")
to create the package...
Now that the package is create you should build and install it.
Let me know if you need any further help.
b
ps: The following page may be helpful:
http://www.biostat.jhsph.edu/~kbroman/Rintro/Rwinpack.html
On Nov 3, 2006, at 10:19 AM, Christophe Poulet wrote:
> Thanks for your answer,
> I've download and install the "oligo" and "makePDpackage", and the
> Zipfiles from Affymetrix with success, but R send another error,
> like this:
> > makePDpackage()
> affymetrix expression
> Error in basename(filein) : argument "designFile" is missing, with
> no default
>
> I tried to find what and where was the design file but I failed to
> find it.
>
> I forget to tell you that I am a Biologist who try to work on
> windows with RGui 2.4.0, it might be important, because I haven't
> got Computer Knowledge. You can find a word doc which contains what
> I have done on R.
>
> Could you tell me what to do, because I don't find this problem in
> the R FAQs for windows.
> Thanks for your time.
> Best regards.
>
> Christophe Poulet
> Laboratoire de Cytog?n?tique, CHU
> Universit? de Li?ge, Sart Tilman
> christophe.poulet at hotmail.com
>
>
> Lancez des recherches en toute s?curit? depuis n'importe quelle
> page Web. T?l?chargez GRATUITEMENT Windows Live Toolbar
> aujourd'hui ! Essayez-le maintenant !
> <r.doc>