ExpressionSet, fitPLM confusion
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Georg Otto ▴ 510
@georg-otto-956
Last seen 10.3 years ago
Dear Bioconductors, Once more, I am a bit confused about an error linked with the new ExpressionSet class. What I am trying to do is to apply fitPLM to an AffyBatch object. First, I generate the AffyBatch object like this: targets<-new("AnnotatedDataFrame") pData(targets)<-read.table("Targets.txt", header=TRUE, row.names=2) Data<-ReadAffy(celfile.path =cel.path, filenames = sampleNames(targets), verbose=TRUE) Then I use fitPLM and get an error: Pset<-fitPLM(Data) Error in .Deprecated(msg = EXPRSET_DEPR_MSG) : unused argument(s) (msg = "The exprSet class is deprecated, use ExpressionSet instead") However ?fitPLM tells me: This function converts an 'AffyBatch' into an 'PLMset' by fitting a specified robust linear model to the probe level data And class() confirms that my object is indeed an AffyBatch: class(Data) [1] "AffyBatch" attr(,"package") [1] "affy" I think I am working with the last versions of the needed libraries (see below). They were updated from the devel repository from source. Any hints? Best, Georg sessionInfo() R version 2.4.0 (2006-10-03) x86_64-redhat-linux-gnu locale: LC_CTYPE=en_US.UTF-8;LC_NUMERIC=C;LC_TIME=en_US.UTF-8;LC_COLLATE=en_US .UTF-8;LC_MONETARY=en_US.UTF-8;LC_MESSAGES=en_US.UTF-8;LC_PAPER=en_US. UTF-8;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US.UTF-8 ;LC_IDENTIFICATION=C attached base packages: [1] "splines" "tools" "stats" "graphics" "grDevices" "utils" [7] "datasets" "methods" "base" other attached packages: zebrafishcdf affyPLM gcrma matchprobes affydata affy "1.15.0" "1.11.8" "2.7.1" "1.7.1" "1.11.1" "1.13.12" affyio Biobase "1.3.1" "1.13.28"
cdf probe gcrma matchprobes affyPLM affyio cdf probe gcrma matchprobes affyPLM affyio • 1.7k views
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@wolfgang-huber-3550
Last seen 4 months ago
EMBL European Molecular Biology Laborat…
Dear Georg, to use the devel versions of Bioconductor (ie what will be Bioconductor 2.0) you will need to use a more recent version of R (what will be R 2.5): the new code in Biobase uses an argument "msg" of the .Deprecated function that was not yet there in R 2.4. See http://www.bioconductor.org/download/BioC2.0/ "BioC 2.0 is currently in development. It is being designed to work with R 2.5.z and is expected to be released in April of 2007." Best wishes Wolfgang Otto wrote: > Dear Bioconductors, > > Once more, I am a bit confused about an error linked with the new > ExpressionSet class. What I am trying to do is to apply fitPLM to an > AffyBatch object. First, I generate the AffyBatch object like this: > > targets<-new("AnnotatedDataFrame") > pData(targets)<-read.table("Targets.txt", header=TRUE, row.names=2) > > > Data<-ReadAffy(celfile.path =cel.path, > filenames = sampleNames(targets), > verbose=TRUE) > > > Then I use fitPLM and get an error: > > Pset<-fitPLM(Data) > > Error in .Deprecated(msg = EXPRSET_DEPR_MSG) : > unused argument(s) (msg = "The exprSet class is deprecated, use ExpressionSet instead") > > However ?fitPLM tells me: > > This function converts an 'AffyBatch' into an 'PLMset' by fitting a > specified robust linear model to the probe level data > > And class() confirms that my object is indeed an AffyBatch: > > class(Data) > [1] "AffyBatch" > attr(,"package") > [1] "affy" > > > I think I am working with the last versions of the needed libraries > (see below). They were updated from the devel repository from > source. Any hints? > > Best, > > Georg > > > sessionInfo() > > R version 2.4.0 (2006-10-03) > x86_64-redhat-linux-gnu > > locale: > LC_CTYPE=en_US.UTF-8;LC_NUMERIC=C;LC_TIME=en_US.UTF-8;LC_COLLATE=en_ US.UTF-8;LC_MONETARY=en_US.UTF-8;LC_MESSAGES=en_US.UTF-8;LC_PAPER=en_U S.UTF-8;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US.UTF -8;LC_IDENTIFICATION=C > > attached base packages: > [1] "splines" "tools" "stats" "graphics" "grDevices" "utils" > [7] "datasets" "methods" "base" > > other attached packages: > zebrafishcdf affyPLM gcrma matchprobes affydata affy > "1.15.0" "1.11.8" "2.7.1" "1.7.1" "1.11.1" "1.13.12" > affyio Biobase > "1.3.1" "1.13.28" > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- ------------------------------------------------------------------ Wolfgang Huber EBI/EMBL Cambridge UK http://www.ebi.ac.uk/huber
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Hi Georg, In addition to what Wolfgang told you, the affyPLM package in the devel repository won't work with R-2.5.0 yet, because some of the changes that have been made to the ExpressionSet object have not been addressed in the affyPLM package. > dat <- ReadAffy() > pset <- fitPLM(dat) Error in checkSlotAssignment(object, name, value) : assignment of an object of class "AnnotatedDataFrame" is not valid for slot "phenoData" in an object of class "PLMset"; is(value, "phenoData") is not TRUE In addition: Warning message: The exprSet class is deprecated, use ExpressionSet instead Your best bet at this time is to go back to using release versions. Best, Jim Wolfgang Huber wrote: > > Dear Georg, > > to use the devel versions of Bioconductor (ie what will be Bioconductor > 2.0) you will need to use a more recent version of R (what will be R > 2.5): the new code in Biobase uses an argument "msg" of the .Deprecated > function that was not yet there in R 2.4. > > See http://www.bioconductor.org/download/BioC2.0/ > "BioC 2.0 is currently in development. It is being designed to work with > R 2.5.z and is expected to be released in April of 2007." > > Best wishes > Wolfgang > > Otto wrote: > >>Dear Bioconductors, >> >>Once more, I am a bit confused about an error linked with the new >>ExpressionSet class. What I am trying to do is to apply fitPLM to an >>AffyBatch object. First, I generate the AffyBatch object like this: >> >>targets<-new("AnnotatedDataFrame") >>pData(targets)<-read.table("Targets.txt", header=TRUE, row.names=2) >> >> >>Data<-ReadAffy(celfile.path =cel.path, >> filenames = sampleNames(targets), >> verbose=TRUE) >> >> >>Then I use fitPLM and get an error: >> >>Pset<-fitPLM(Data) >> >>Error in .Deprecated(msg = EXPRSET_DEPR_MSG) : >> unused argument(s) (msg = "The exprSet class is deprecated, use ExpressionSet instead") >> >>However ?fitPLM tells me: >> >>This function converts an 'AffyBatch' into an 'PLMset' by fitting a >>specified robust linear model to the probe level data >> >>And class() confirms that my object is indeed an AffyBatch: >> >>class(Data) >>[1] "AffyBatch" >>attr(,"package") >>[1] "affy" >> >> >>I think I am working with the last versions of the needed libraries >>(see below). They were updated from the devel repository from >>source. Any hints? >> >>Best, >> >>Georg >> >> >>sessionInfo() >> >>R version 2.4.0 (2006-10-03) >>x86_64-redhat-linux-gnu >> >>locale: >>LC_CTYPE=en_US.UTF-8;LC_NUMERIC=C;LC_TIME=en_US.UTF-8;LC_COLLATE=en_ US.UTF-8;LC_MONETARY=en_US.UTF-8;LC_MESSAGES=en_US.UTF-8;LC_PAPER=en_U S.UTF-8;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US.UTF -8;LC_IDENTIFICATION=C >> >>attached base packages: >>[1] "splines" "tools" "stats" "graphics" "grDevices" "utils" >>[7] "datasets" "methods" "base" >> >>other attached packages: >>zebrafishcdf affyPLM gcrma matchprobes affydata affy >> "1.15.0" "1.11.8" "2.7.1" "1.7.1" "1.11.1" "1.13.12" >> affyio Biobase >> "1.3.1" "1.13.28" >> >>_______________________________________________ >>Bioconductor mailing list >>Bioconductor at stat.math.ethz.ch >>https://stat.ethz.ch/mailman/listinfo/bioconductor >>Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > > -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues.
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Hi, maybe a workaround would be to have a copy of the new version of .Deprecated() in the global environment. Here it is: .Deprecated <- function (new, package = NULL, msg) { if (missing(msg)) { msg <- gettextf("'%s' is deprecated.\n", as.character(sys.call(sys.parent())[[1]])) if (!missing(new)) msg <- c(msg, gettextf("Use '%s' instead.\n", new)) msg <- c(msg, if (!is.null(package)) gettextf("See help(\"Deprecated\") and help(\"%s-deprecated\").", package) else gettext("See help(\"Deprecated\")")) } else msg = as.character(msg) warning(paste(msg, collapse = ""), call. = FALSE, domain = NA) } If you don't bother with the 'msg' you could even do: .Deprecated <- function(..., msg) base::.Deprecated(...) I haven't tried it with your example, though. /Henrik On 12/15/06, James W. MacDonald <jmacdon at="" med.umich.edu=""> wrote: > Hi Georg, > > In addition to what Wolfgang told you, the affyPLM package in the devel > repository won't work with R-2.5.0 yet, because some of the changes that > have been made to the ExpressionSet object have not been addressed in > the affyPLM package. > > > dat <- ReadAffy() > > pset <- fitPLM(dat) > Error in checkSlotAssignment(object, name, value) : > assignment of an object of class "AnnotatedDataFrame" is not valid for > slot "phenoData" in an object of class "PLMset"; is(value, "phenoData") > is not TRUE > In addition: Warning message: > The exprSet class is deprecated, use ExpressionSet instead > > Your best bet at this time is to go back to using release versions. > > Best, > > Jim > > > Wolfgang Huber wrote: > > > > Dear Georg, > > > > to use the devel versions of Bioconductor (ie what will be Bioconductor > > 2.0) you will need to use a more recent version of R (what will be R > > 2.5): the new code in Biobase uses an argument "msg" of the .Deprecated > > function that was not yet there in R 2.4. > > > > See http://www.bioconductor.org/download/BioC2.0/ > > "BioC 2.0 is currently in development. It is being designed to work with > > R 2.5.z and is expected to be released in April of 2007." > > > > Best wishes > > Wolfgang > > > > Otto wrote: > > > >>Dear Bioconductors, > >> > >>Once more, I am a bit confused about an error linked with the new > >>ExpressionSet class. What I am trying to do is to apply fitPLM to an > >>AffyBatch object. First, I generate the AffyBatch object like this: > >> > >>targets<-new("AnnotatedDataFrame") > >>pData(targets)<-read.table("Targets.txt", header=TRUE, row.names=2) > >> > >> > >>Data<-ReadAffy(celfile.path =cel.path, > >> filenames = sampleNames(targets), > >> verbose=TRUE) > >> > >> > >>Then I use fitPLM and get an error: > >> > >>Pset<-fitPLM(Data) > >> > >>Error in .Deprecated(msg = EXPRSET_DEPR_MSG) : > >> unused argument(s) (msg = "The exprSet class is deprecated, use ExpressionSet instead") > >> > >>However ?fitPLM tells me: > >> > >>This function converts an 'AffyBatch' into an 'PLMset' by fitting a > >>specified robust linear model to the probe level data > >> > >>And class() confirms that my object is indeed an AffyBatch: > >> > >>class(Data) > >>[1] "AffyBatch" > >>attr(,"package") > >>[1] "affy" > >> > >> > >>I think I am working with the last versions of the needed libraries > >>(see below). They were updated from the devel repository from > >>source. Any hints? > >> > >>Best, > >> > >>Georg > >> > >> > >>sessionInfo() > >> > >>R version 2.4.0 (2006-10-03) > >>x86_64-redhat-linux-gnu > >> > >>locale: > >>LC_CTYPE=en_US.UTF-8;LC_NUMERIC=C;LC_TIME=en_US.UTF-8;LC_COLLATE=e n_US.UTF-8;LC_MONETARY=en_US.UTF-8;LC_MESSAGES=en_US.UTF-8;LC_PAPER=en _US.UTF-8;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US.U TF-8;LC_IDENTIFICATION=C > >> > >>attached base packages: > >>[1] "splines" "tools" "stats" "graphics" "grDevices" "utils" > >>[7] "datasets" "methods" "base" > >> > >>other attached packages: > >>zebrafishcdf affyPLM gcrma matchprobes affydata affy > >> "1.15.0" "1.11.8" "2.7.1" "1.7.1" "1.11.1" "1.13.12" > >> affyio Biobase > >> "1.3.1" "1.13.28" > >> > >>_______________________________________________ > >>Bioconductor mailing list > >>Bioconductor at stat.math.ethz.ch > >>https://stat.ethz.ch/mailman/listinfo/bioconductor > >>Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > > > > > > > > -- > James W. MacDonald, M.S. > Biostatistician > Affymetrix and cDNA Microarray Core > University of Michigan Cancer Center > 1500 E. Medical Center Drive > 7410 CCGC > Ann Arbor MI 48109 > 734-647-5623 > > > ********************************************************** > Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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