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Jenny Drnevich
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@jenny-drnevich-382
Last seen 10.4 years ago
Hi Benilton & all,
I've gotten some data from Affy's mouse promoter 1.0R tiling array,
and I'm
trying to figure out how to analyze it. I've looked in the archives,
and
found some of your posts about the oligo and makePlatformDesign
packages
very helpful:
https://stat.ethz.ch/pipermail/bioconductor/2007-January/015655.html
https://stat.ethz.ch/pipermail/bioconductor/2006-August/014167.html
My question: what's the meaning of the different arguments for
makePDpackage? In your tutorial slides from BioC2006, you show it
called
along these lines:
makePDpackage("Mm_PromPR_v01_NCBIv35.bpmap", type="tiling",
genomebuild="mg33")
However, in the August post above, it was being used this way:
makePDpackage("Hs_PromPF_v02-3_NCBIv34.bpmap",
file1="Hs_PromPF_v02.cif",
manufacturer="affymetrix", type="tiling")
What is the file1 argument? The help page doesn't say what these
should be
for tiling arrays, and it appears from your tutorial that it's not
necessary. For the mouse promoter tiling array, there are two .cif
files,
one forward and one reverse. Here's what Affy's readme file said about
them: "The R and F tiling sets have been split into two separate zip
files
so make sure you have the right file."
Should I be using these? How should I be using these? My first goal is
just
to look at the raw data values, and there are only 2 arrays - one CHip
and
one control. The researchers want see how well these arrays turned
out, and
get some idea on what level of replication may be needed. And if
anyone
knows any good articles on analyzing CHip-on-chip arrays, please point
them
out to me - I've been finding some, but I would like to know what
others
have found useful.
Thanks,
Jenny
Jenny Drnevich, Ph.D.
Functional Genomics Bioinformatics Specialist
W.M. Keck Center for Comparative and Functional Genomics
Roy J. Carver Biotechnology Center
University of Illinois, Urbana-Champaign
330 ERML
1201 W. Gregory Dr.
Urbana, IL 61801
USA
ph: 217-244-7355
fax: 217-265-5066
e-mail: drnevich at uiuc.edu