Renaming row names (U133A/B joining problem)
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Daniel Brewer ★ 1.9k
@daniel-brewer-1791
Last seen 9.7 years ago
Hi, I have a expression dataset where I want to join Affymetrix U133A and B arrays. The rownames of the expression matrix are set to the probe names. To do this I want to treat all the probe names that are shared between the arrays as unique by appending an "_A" or "_B". I do this by finding the names of the probe that are shared and then running: rownames(expr.matrix[match(ProbeIntersect,rownames(expr.matrix)),]) <- paste(rownames(expr.matrix[match(ProbeIntersect,rownames(expr.matrix)) ,]),"_A",sep="") Where "expr.matrix" is the expression matrix and "ProbeIntersect" is the list of probe names that are shared. This command though does not seem to change the row names. Any idea what I am doing wrong? Thanks Daniel -- ************************************************************** Daniel Brewer, Ph.D. Institute of Cancer Research Email: daniel.brewer at icr.ac.uk ************************************************************** The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addre...{{dropped}}
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@martin-morgan-1513
Last seen 26 days ago
United States
Hi Daniel, I think the basic syntax is inverted -- rownames(obj)[1:3] <- 3:1 instead of rownames(obj[1:3,]) <- 3:1 It's a little hard to tell what expr.matrix is. A matrix? > m <- matrix(0,10,5) > rownames(m)[1:4] <- 4:1 # need to have dims before they can be changed! Error in dimnames(x) <- dn : length of 'dimnames' [1] not equal to array extent > rownames(m) <- 1:10 > rownames(m)[1:4] <- 4:1 > m [,1] [,2] [,3] [,4] [,5] 4 0 0 0 0 0 3 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 5 0 0 0 0 0 6 0 0 0 0 0 7 0 0 0 0 0 8 0 0 0 0 0 9 0 0 0 0 0 10 0 0 0 0 0 But maybe it's an 'ExpressionSet' or 'exprSet'. What does > class(expr.matrix) say? If it's an ExpressionSet (or I think also an exprSet), then you'll want to do the equivalent of > obj <- new("ExpressionSet", exprs=matrix(0,10,5)) > obj ExpressionSet (storageMode: lockedEnvironment) assayData: 10 features, 5 samples element names: exprs phenoData sampleNames: 1, 2, ..., 5 (5 total) varLabels and varMetadata: none featureData featureNames: 1, 2, ..., 10 (10 total) varLabels and varMetadata: none experimentData: use 'experimentData(object)' Annotation character(0) > featureNames(obj) [1] "1" "2" "3" "4" "5" "6" "7" "8" "9" "10" > featureNames(obj)[2:4] <- 4:2 > featureNames(obj) [1] "1" "4" "3" "2" "5" "6" "7" "8" "9" "10" > obj ExpressionSet (storageMode: lockedEnvironment) assayData: 10 features, 5 samples element names: exprs phenoData sampleNames: 1, 2, ..., 5 (5 total) varLabels and varMetadata: none featureData featureNames: 1, 4, ..., 10 (10 total) varLabels and varMetadata: none experimentData: use 'experimentData(object)' Annotation character(0) Hope that helps, Martin Daniel Brewer <daniel.brewer at="" icr.ac.uk=""> writes: > Hi, > > I have a expression dataset where I want to join Affymetrix U133A and B > arrays. The rownames of the expression matrix are set to the probe > names. To do this I want to treat all the probe names that are shared > between the arrays as unique by appending an "_A" or "_B". I do this by > finding the names of the probe that are shared and then running: > > rownames(expr.matrix[match(ProbeIntersect,rownames(expr.matrix)),]) <- > paste(rownames(expr.matrix[match(ProbeIntersect,rownames(expr.matrix )),]),"_A",sep="") > > Where "expr.matrix" is the expression matrix and "ProbeIntersect" is the > list of probe names that are shared. This command though does not seem > to change the row names. Any idea what I am doing wrong? > > Thanks > > Daniel > > -- > ************************************************************** > > Daniel Brewer, Ph.D. > Institute of Cancer Research > Email: daniel.brewer at icr.ac.uk > > ************************************************************** > > The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. > > This e-mail message is confidential and for use by the addre...{{dropped}} > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Martin Morgan Bioconductor / Computational Biology http://bioconductor.org
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expr.matrix is a matrix and it did work when I inverted the syntax. Many thanks Daniel Martin Morgan wrote: > Hi Daniel, > > I think the basic syntax is inverted -- > > rownames(obj)[1:3] <- 3:1 > > instead of > > rownames(obj[1:3,]) <- 3:1 > > It's a little hard to tell what expr.matrix is. A matrix? > >> m <- matrix(0,10,5) >> rownames(m)[1:4] <- 4:1 # need to have dims before they can be changed! > Error in dimnames(x) <- dn : length of 'dimnames' [1] not equal to array extent >> rownames(m) <- 1:10 >> rownames(m)[1:4] <- 4:1 >> m > [,1] [,2] [,3] [,4] [,5] > 4 0 0 0 0 0 > 3 0 0 0 0 0 > 2 0 0 0 0 0 > 1 0 0 0 0 0 > 5 0 0 0 0 0 > 6 0 0 0 0 0 > 7 0 0 0 0 0 > 8 0 0 0 0 0 > 9 0 0 0 0 0 > 10 0 0 0 0 0 > > > But maybe it's an 'ExpressionSet' or 'exprSet'. What does > >> class(expr.matrix) > > say? If it's an ExpressionSet (or I think also an exprSet), then > you'll want to do the equivalent of > >> obj <- new("ExpressionSet", exprs=matrix(0,10,5)) >> obj > ExpressionSet (storageMode: lockedEnvironment) > assayData: 10 features, 5 samples > element names: exprs > phenoData > sampleNames: 1, 2, ..., 5 (5 total) > varLabels and varMetadata: none > featureData > featureNames: 1, 2, ..., 10 (10 total) > varLabels and varMetadata: none > experimentData: use 'experimentData(object)' > Annotation character(0) >> featureNames(obj) > [1] "1" "2" "3" "4" "5" "6" "7" "8" "9" "10" >> featureNames(obj)[2:4] <- 4:2 >> featureNames(obj) > [1] "1" "4" "3" "2" "5" "6" "7" "8" "9" "10" >> obj > ExpressionSet (storageMode: lockedEnvironment) > assayData: 10 features, 5 samples > element names: exprs > phenoData > sampleNames: 1, 2, ..., 5 (5 total) > varLabels and varMetadata: none > featureData > featureNames: 1, 4, ..., 10 (10 total) > varLabels and varMetadata: none > experimentData: use 'experimentData(object)' > Annotation character(0) > > Hope that helps, > > Martin > > > Daniel Brewer <daniel.brewer at="" icr.ac.uk=""> writes: > >> Hi, >> >> I have a expression dataset where I want to join Affymetrix U133A and B >> arrays. The rownames of the expression matrix are set to the probe >> names. To do this I want to treat all the probe names that are shared >> between the arrays as unique by appending an "_A" or "_B". I do this by >> finding the names of the probe that are shared and then running: >> >> rownames(expr.matrix[match(ProbeIntersect,rownames(expr.matrix)),]) <- >> paste(rownames(expr.matrix[match(ProbeIntersect,rownames(expr.matri x)),]),"_A",sep="") >> >> Where "expr.matrix" is the expression matrix and "ProbeIntersect" is the >> list of probe names that are shared. This command though does not seem >> to change the row names. Any idea what I am doing wrong? >> >> Thanks >> >> Daniel >> -- ************************************************************** Daniel Brewer, Ph.D. Institute of Cancer Research Email: daniel.brewer at icr.ac.uk ************************************************************** The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addre...{{dropped}}
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