Affymetrix Tiling arrays
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@alessandro-ferrantini-2030
Last seen 10.2 years ago
Hi everyone, I am starting to analyse some tiling arrays, but I cannot find the corresponding CDF file in bioconductor (for example hs35bm03rv02cdf or hs35bm03rv02cdf). What I am exactly looking for is to know the position (or index) of the perfect matches and the corresponding sequence. Is there any way to do it? Thank you in advance, Alessandro ______________________________________________________________________ ______________ Finding fabulous fares is fun.
cdf cdf • 817 views
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@benilton-carvalho-1375
Last seen 4.7 years ago
Brazil/Campinas/UNICAMP
The tiling arrays do not have a CDF, they use a BPMAP instead, which can be used in conjunction with the makePlatformDesign package to create a data package, which is in turn used by the oligo package to read in the CEL files. How many samples are you going to be working with? This will require you a good amount of memory.... b On Apr 3, 2007, at 10:07 AM, Alessandro Ferrantini wrote: > Hi everyone, > I am starting to analyse some tiling arrays, but I > cannot find the corresponding CDF file in bioconductor (for example > hs35bm03rv02cdf or hs35bm03rv02cdf). > What I am exactly looking for is to know the position (or index) of > the > perfect matches and the corresponding sequence. Is there any way to do > it? > > Thank you in advance, > > Alessandro > > > > > ______________________________________________________________________ > ______________ > Finding fabulous fares is fun. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor
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@alessandro-ferrantini-2030
Last seen 10.2 years ago
Dear Benilton, Thanks a lot for your suggestion. I've installed R version 2.5 and makePlatformDesign and oligo packages. I've tried the following: > makePDpackage("Hs35b_M01R_v01_NCBIv35.bpmap",type="tiling",genomebui ld="hg15") but I got an error: affymetrix tiling Error in makePDpackage("Hs35b_M01R_v01_NCBIv35.bpmap", type = "tiling", : could not find function "cleanPlatformName" Any ideas? Here below the sessionInfo. Thank you, Alessandro > sessionInfo() R version 2.5.0 alpha (2007-04-01 r41004) i686-pc-linux-gnu locale: LC_CTYPE=en_US.UTF-8;LC_NUMERIC=C;LC_TIME=en_US.UTF-8;LC_COLLATE=en_US .UTF-8;LC_MONETARY=en_US.UTF-8;LC_MESSAGES=en_US.UTF-8;LC_PAPER=en_US. UTF-8;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US.UTF-8 ;LC_IDENTIFICATION=C attached base packages: [1] "splines" "tools" "stats" "graphics" "grDevices" "utils" [7] "datasets" "methods" "base" other attached packages: makePlatformDesign oligo BufferedMatrixMethods "0.99.24" "0.99.96" "0.1.1" BufferedMatrix RSQLite DBI "0.1.29" "0.5-3" "0.2-1" Biobase affyio "1.13.47" "1.3.3" ______________________________________________________________________ ______________ The fish are biting.
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the fix should appear later today at: http://www.bioconductor.org/packages/2.0/bioc/html/ makePlatformDesign.html v 0.99.25 b On Apr 4, 2007, at 1:28 PM, Alessandro Ferrantini wrote: > Dear Benilton, > > Thanks a lot for your suggestion. I've installed R version 2.5 and > makePlatformDesign and oligo packages. I've tried the following: > > > >> makePDpackage >> ("Hs35b_M01R_v01_NCBIv35.bpmap",type="tiling",genomebuild="hg15") > > > > but I got an error: > > > > affymetrix tiling > > Error in makePDpackage("Hs35b_M01R_v01_NCBIv35.bpmap", type = > "tiling", : > > could not find function "cleanPlatformName" > > > > Any ideas? Here below the sessionInfo. > > Thank you, > > Alessandro > > > > > > > >> sessionInfo() > > R version 2.5.0 alpha (2007-04-01 r41004) > > i686-pc-linux-gnu > > > > locale: > > LC_CTYPE=en_US.UTF-8;LC_NUMERIC=C;LC_TIME=en_US.UTF-8;LC_COLLATE=en_US > .UTF-8;LC_MONETARY=en_US.UTF-8;LC_MESSAGES=en_US.UTF-8;LC_PAPER=en_US. > UTF-8;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US.UTF-8 > ;LC_IDENTIFICATION=C > > > > attached base packages: > > [1] "splines" "tools" "stats" "graphics" "grDevices" > "utils" > > [7] "datasets" "methods" "base" > > > > other attached packages: > > makePlatformDesign oligo BufferedMatrixMethods > > "0.99.24" "0.99.96" "0.1.1" > > BufferedMatrix RSQLite DBI > > "0.1.29" "0.5-3" "0.2-1" > > Biobase affyio > > "1.13.47" "1.3.3" > > > > > > ______________________________________________________________________ > ______________ > The fish are biting. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor
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