how to do the following using justRMA in affy package?
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@james-anderson-1641
Last seen 10.3 years ago
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Seth Falcon ★ 7.4k
@seth-falcon-992
Last seen 10.3 years ago
James Anderson <janderson_net at="" yahoo.com=""> writes: > Hi, > > I have 100 cel files (U133A) in a folder, I want to randomly select 70 > cel files from them and use justRMA to get the normalized intensity. I > need to do this 50 times (every time, the 70 cel files are not > necessarily the same). More imporantly, I want to save the expression > value obtained by justRMA to file name Data_1.txt Data_2.txt > Data_3.txt, ..., Data_50.txt in an automatic fashion. I tried myself > first and could not figure out how to do it, could anybody offer me > any help or suggestions? Try reading the help pages for these functions: list.files sample save paste for (i in 1:50) { Create a character vector of your 100 CEL files. Sample 70 elements. Run justRMA save to file paste("data_", i, ".txt", sep="") } + seth -- Seth Falcon | Computational Biology | Fred Hutchinson Cancer Research Center http://bioconductor.org
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@james-w-macdonald-5106
Last seen 12 hours ago
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James Anderson wrote: > Hi, > > I have 100 cel files (U133A) in a folder, I want to randomly select > 70 cel files from them and use justRMA to get the normalized > intensity. I need to do this 50 times (every time, the 70 cel files > are not necessarily the same). More imporantly, I want to save the > expression value obtained by justRMA to file name Data_1.txt > Data_2.txt Data_3.txt, ..., Data_50.txt in an automatic fashion. I > tried myself first and could not figure out how to do it, could > anybody offer me any help or suggestions? library(affy) for(i in 1:50){ ind <- sample(1:100, 70) eset <- justRMA(filenames=list.celfiles()[ind]) write.exprs(eset, paste("Data_", i, ".txt", sep=""), col.names=NA) } Best, Jim > > Many thanks, > > James > > > --------------------------------- > > > [[alternative HTML version deleted]] > > _______________________________________________ Bioconductor mailing > list Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor Search the > archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues.
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