pmStrand() in oligo (version...)
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@de-bondt-an-7114-prdbe-1572
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@benilton-carvalho-1375
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Hi An, I fixed that problem, thanks for the report. Also, regarding the .crlmm.regions packages, these are not anymore required. If you use the following version instead (which should appear soon on BioC), these problems should be taken care of: http://www.biostat.jhsph.edu/~bcarvalh/oligo_1.0.2-3.tar.gz Thank you, b On Jun 1, 2007, at 5:10 AM, De Bondt, An-7114 [PRDBE] wrote: > Dear useRs, > > I am looking into the raw data of the 500K SNP arrays and I would > like to > select the data from only one strand, sense OR antisense. How > could I do > this? Probably something like: > > fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) > strand.sty <- pmStrand(get(annotation(fsetSty))) > > but the last command results in "Error: could not find function > "pmStrand"" > although ?pmStrand is recognised and gives... > > Description: > Returns the strand information on SNP arrays for PM probes (0 - > sense / 1 - antisense). > Usage: > pmStrand(object) > Arguments: > object: 'AffySNPPDInfo' object > > > > Thanks in advance for your help! > An > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor
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@de-bondt-an-7114-prdbe-1572
Last seen 9.7 years ago
Hi Ben, When using oligo version 1.0.2-3, the pmStrand function indeed works :-) but the snprma function results in an error: fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) rmaSty <- snprma(fsetSty) Normalizing...Error in readChar(con, 5) : cannot open the connection In addition: Warning message: cannot open compressed file How can I check which connection needs to be established? Thanks in advance for your help! An -----Original Message----- From: Benilton Carvalho [mailto:bcarvalh@jhsph.edu] Sent: Friday, 1 June 2007 15:30 To: De Bondt, An-7114 [PRDBE] Cc: bioconductor at stat.math.ethz.ch Subject: Re: [BioC] pmStrand() in oligo (version...) Hi An, I fixed that problem, thanks for the report. Also, regarding the .crlmm.regions packages, these are not anymore required. If you use the following version instead (which should appear soon on BioC), these problems should be taken care of: http://www.biostat.jhsph.edu/~bcarvalh/oligo_1.0.2-3.tar.gz Thank you, b On Jun 1, 2007, at 5:10 AM, De Bondt, An-7114 [PRDBE] wrote: > Dear useRs, > > I am looking into the raw data of the 500K SNP arrays and I would > like to > select the data from only one strand, sense OR antisense. How > could I do > this? Probably something like: > > fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) > strand.sty <- pmStrand(get(annotation(fsetSty))) > > but the last command results in "Error: could not find function > "pmStrand"" > although ?pmStrand is recognised and gives... > > Description: > Returns the strand information on SNP arrays for PM probes (0 - > sense / 1 - antisense). > Usage: > pmStrand(object) > Arguments: > object: 'AffySNPPDInfo' object > > > > Thanks in advance for your help! > An > > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor
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Hi An, that's problably R complaining a file that it didn't find. The strange thing is that I can't reproduce it here. Could you please forward me the results of traceback()? This would happen if, for example, oligo finds the old version of the pd.mapping250k.sty package. b On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: > Hi Ben, > > When using oligo version 1.0.2-3, the pmStrand function indeed > works :-) > but the snprma function results in an error: > > fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) > rmaSty <- snprma(fsetSty) > > Normalizing...Error in readChar(con, 5) : cannot open the connection > In addition: Warning message: > cannot open compressed file > > How can I check which connection needs to be established? > > Thanks in advance for your help! > An > > -----Original Message----- > From: Benilton Carvalho [mailto:bcarvalh at jhsph.edu] > Sent: Friday, 1 June 2007 15:30 > To: De Bondt, An-7114 [PRDBE] > Cc: bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] pmStrand() in oligo (version...) > > > Hi An, > > I fixed that problem, thanks for the report. > > Also, regarding the .crlmm.regions packages, these are not anymore > required. > > If you use the following version instead (which should appear soon on > BioC), these problems should be taken care of: > > http://www.biostat.jhsph.edu/~bcarvalh/oligo_1.0.2-3.tar.gz > > Thank you, > > b > > On Jun 1, 2007, at 5:10 AM, De Bondt, An-7114 [PRDBE] wrote: > >> Dear useRs, >> >> I am looking into the raw data of the 500K SNP arrays and I would >> like to >> select the data from only one strand, sense OR antisense. How >> could I do >> this? Probably something like: >> >> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >> strand.sty <- pmStrand(get(annotation(fsetSty))) >> >> but the last command results in "Error: could not find function >> "pmStrand"" >> although ?pmStrand is recognised and gives... >> >> Description: >> Returns the strand information on SNP arrays for PM probes (0 - >> sense / 1 - antisense). >> Usage: >> pmStrand(object) >> Arguments: >> object: 'AffySNPPDInfo' object >> >> >> >> Thanks in advance for your help! >> An >> >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/ >> gmane.science.biology.informatics.conductor
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@de-bondt-an-7114-prdbe-1572
Last seen 9.7 years ago
Hi Ben, Here some extra info, hopefully useful to pinpoint the issue: > rmaSty <- snprma(fsetSty) This may take a while. Adjusting for sequence and fragment length....... done. Normalizing...Error in readChar(con, 5) : cannot open the connection In addition: Warning message: cannot open compressed file '' > traceback() 3: readChar(con, 5) 2: load(system.file("extdata", paste(annot, "Ref.rda", sep = ""), package = annot)) 1: snprma(fsetSty) > package.version("pd.mapping250k.sty") [1] "0.2.5" Cheers, An -----Original Message----- From: Benilton Carvalho [mailto:bcarvalh@jhsph.edu] Sent: Monday, 4 June 2007 17:02 To: De Bondt, An-7114 [PRDBE] Cc: bioconductor at stat.math.ethz.ch Subject: Re: [BioC] pmStrand() in oligo (version...) Hi An, that's problably R complaining a file that it didn't find. The strange thing is that I can't reproduce it here. Could you please forward me the results of traceback()? This would happen if, for example, oligo finds the old version of the pd.mapping250k.sty package. b On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: > Hi Ben, > > When using oligo version 1.0.2-3, the pmStrand function indeed > works :-) > but the snprma function results in an error: > > fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) > rmaSty <- snprma(fsetSty) > > Normalizing...Error in readChar(con, 5) : cannot open the connection > In addition: Warning message: > cannot open compressed file > > How can I check which connection needs to be established? > > Thanks in advance for your help! > An > > -----Original Message----- > From: Benilton Carvalho [mailto:bcarvalh at jhsph.edu] > Sent: Friday, 1 June 2007 15:30 > To: De Bondt, An-7114 [PRDBE] > Cc: bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] pmStrand() in oligo (version...) > > > Hi An, > > I fixed that problem, thanks for the report. > > Also, regarding the .crlmm.regions packages, these are not anymore > required. > > If you use the following version instead (which should appear soon on > BioC), these problems should be taken care of: > > http://www.biostat.jhsph.edu/~bcarvalh/oligo_1.0.2-3.tar.gz > > Thank you, > > b > > On Jun 1, 2007, at 5:10 AM, De Bondt, An-7114 [PRDBE] wrote: > >> Dear useRs, >> >> I am looking into the raw data of the 500K SNP arrays and I would >> like to >> select the data from only one strand, sense OR antisense. How >> could I do >> this? Probably something like: >> >> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >> strand.sty <- pmStrand(get(annotation(fsetSty))) >> >> but the last command results in "Error: could not find function >> "pmStrand"" >> although ?pmStrand is recognised and gives... >> >> Description: >> Returns the strand information on SNP arrays for PM probes (0 - >> sense / 1 - antisense). >> Usage: >> pmStrand(object) >> Arguments: >> object: 'AffySNPPDInfo' object >> >> >> >> Thanks in advance for your help! >> An >> >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/ >> gmane.science.biology.informatics.conductor
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That's strange An. Could you please tell us what you get with: dir(system.file("extdata", package = "pd.mapping250k.sty")) I wonder if there was a problem when you installed the pd.mapping package, as it (v. 0.2.5) does contain the Ref file application is complaining about... cheers, b On Jun 5, 2007, at 2:22 AM, De Bondt, An-7114 [PRDBE] wrote: > Hi Ben, > > Here some extra info, hopefully useful to pinpoint the issue: > >> rmaSty <- snprma(fsetSty) > This may take a while. > Adjusting for sequence and fragment length....... done. > Normalizing...Error in readChar(con, 5) : cannot open the > connection > In addition: Warning message: > cannot open compressed file '' >> traceback() > 3: readChar(con, 5) > 2: load(system.file("extdata", paste(annot, "Ref.rda", sep = ""), > package = annot)) > 1: snprma(fsetSty) >> package.version("pd.mapping250k.sty") > [1] "0.2.5" > > > Cheers, > An > > > -----Original Message----- > From: Benilton Carvalho [mailto:bcarvalh at jhsph.edu] > Sent: Monday, 4 June 2007 17:02 > To: De Bondt, An-7114 [PRDBE] > Cc: bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] pmStrand() in oligo (version...) > > > Hi An, > > that's problably R complaining a file that it didn't find. The > strange thing is that I can't reproduce it here. Could you please > forward me the results of traceback()? > > This would happen if, for example, oligo finds the old version of the > pd.mapping250k.sty package. > > b > > On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: > >> Hi Ben, >> >> When using oligo version 1.0.2-3, the pmStrand function indeed >> works :-) >> but the snprma function results in an error: >> >> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >> rmaSty <- snprma(fsetSty) >> >> Normalizing...Error in readChar(con, 5) : cannot open the connection >> In addition: Warning message: >> cannot open compressed file >> >> How can I check which connection needs to be established? >> >> Thanks in advance for your help! >> An >> >> -----Original Message----- >> From: Benilton Carvalho [mailto:bcarvalh at jhsph.edu] >> Sent: Friday, 1 June 2007 15:30 >> To: De Bondt, An-7114 [PRDBE] >> Cc: bioconductor at stat.math.ethz.ch >> Subject: Re: [BioC] pmStrand() in oligo (version...) >> >> >> Hi An, >> >> I fixed that problem, thanks for the report. >> >> Also, regarding the .crlmm.regions packages, these are not anymore >> required. >> >> If you use the following version instead (which should appear soon on >> BioC), these problems should be taken care of: >> >> http://www.biostat.jhsph.edu/~bcarvalh/oligo_1.0.2-3.tar.gz >> >> Thank you, >> >> b >> >> On Jun 1, 2007, at 5:10 AM, De Bondt, An-7114 [PRDBE] wrote: >> >>> Dear useRs, >>> >>> I am looking into the raw data of the 500K SNP arrays and I would >>> like to >>> select the data from only one strand, sense OR antisense. How >>> could I do >>> this? Probably something like: >>> >>> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >>> strand.sty <- pmStrand(get(annotation(fsetSty))) >>> >>> but the last command results in "Error: could not find function >>> "pmStrand"" >>> although ?pmStrand is recognised and gives... >>> >>> Description: >>> Returns the strand information on SNP arrays for PM probes (0 - >>> sense / 1 - antisense). >>> Usage: >>> pmStrand(object) >>> Arguments: >>> object: 'AffySNPPDInfo' object >>> >>> >>> >>> Thanks in advance for your help! >>> An >>> >>> >>> [[alternative HTML version deleted]] >>> >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at stat.math.ethz.ch >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: http://news.gmane.org/ >>> gmane.science.biology.informatics.conductor
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@de-bondt-an-7114-prdbe-1572
Last seen 9.7 years ago
Hi Ben, > dir(system.file("extdata", package = "pd.mapping250k.sty")) [1] "pd.mapping250k.sty.sqlite" "seqMat.rda" Is this the output what it should be? An -----Original Message----- From: Benilton Carvalho [mailto:bcarvalh@jhsph.edu] Sent: Tuesday, 5 June 2007 8:34 To: De Bondt, An-7114 [PRDBE] Cc: bioconductor at stat.math.ethz.ch Subject: Re: [BioC] pmStrand() in oligo (version...) That's strange An. Could you please tell us what you get with: dir(system.file("extdata", package = "pd.mapping250k.sty")) I wonder if there was a problem when you installed the pd.mapping package, as it (v. 0.2.5) does contain the Ref file application is complaining about... cheers, b On Jun 5, 2007, at 2:22 AM, De Bondt, An-7114 [PRDBE] wrote: > Hi Ben, > > Here some extra info, hopefully useful to pinpoint the issue: > >> rmaSty <- snprma(fsetSty) > This may take a while. > Adjusting for sequence and fragment length....... done. > Normalizing...Error in readChar(con, 5) : cannot open the > connection > In addition: Warning message: > cannot open compressed file '' >> traceback() > 3: readChar(con, 5) > 2: load(system.file("extdata", paste(annot, "Ref.rda", sep = ""), > package = annot)) > 1: snprma(fsetSty) >> package.version("pd.mapping250k.sty") > [1] "0.2.5" > > > Cheers, > An > > > -----Original Message----- > From: Benilton Carvalho [mailto:bcarvalh at jhsph.edu] > Sent: Monday, 4 June 2007 17:02 > To: De Bondt, An-7114 [PRDBE] > Cc: bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] pmStrand() in oligo (version...) > > > Hi An, > > that's problably R complaining a file that it didn't find. The > strange thing is that I can't reproduce it here. Could you please > forward me the results of traceback()? > > This would happen if, for example, oligo finds the old version of the > pd.mapping250k.sty package. > > b > > On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: > >> Hi Ben, >> >> When using oligo version 1.0.2-3, the pmStrand function indeed >> works :-) >> but the snprma function results in an error: >> >> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >> rmaSty <- snprma(fsetSty) >> >> Normalizing...Error in readChar(con, 5) : cannot open the connection >> In addition: Warning message: >> cannot open compressed file >> >> How can I check which connection needs to be established? >> >> Thanks in advance for your help! >> An >> >> -----Original Message----- >> From: Benilton Carvalho [mailto:bcarvalh at jhsph.edu] >> Sent: Friday, 1 June 2007 15:30 >> To: De Bondt, An-7114 [PRDBE] >> Cc: bioconductor at stat.math.ethz.ch >> Subject: Re: [BioC] pmStrand() in oligo (version...) >> >> >> Hi An, >> >> I fixed that problem, thanks for the report. >> >> Also, regarding the .crlmm.regions packages, these are not anymore >> required. >> >> If you use the following version instead (which should appear soon on >> BioC), these problems should be taken care of: >> >> http://www.biostat.jhsph.edu/~bcarvalh/oligo_1.0.2-3.tar.gz >> >> Thank you, >> >> b >> >> On Jun 1, 2007, at 5:10 AM, De Bondt, An-7114 [PRDBE] wrote: >> >>> Dear useRs, >>> >>> I am looking into the raw data of the 500K SNP arrays and I would >>> like to >>> select the data from only one strand, sense OR antisense. How >>> could I do >>> this? Probably something like: >>> >>> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >>> strand.sty <- pmStrand(get(annotation(fsetSty))) >>> >>> but the last command results in "Error: could not find function >>> "pmStrand"" >>> although ?pmStrand is recognised and gives... >>> >>> Description: >>> Returns the strand information on SNP arrays for PM probes (0 - >>> sense / 1 - antisense). >>> Usage: >>> pmStrand(object) >>> Arguments: >>> object: 'AffySNPPDInfo' object >>> >>> >>> >>> Thanks in advance for your help! >>> An >>> >>> >>> [[alternative HTML version deleted]] >>> >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at stat.math.ethz.ch >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: http://news.gmane.org/ >>> gmane.science.biology.informatics.conductor
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