Question: Normalization of 2 color Nimblegen array
gravatar for dhruv pant
12.5 years ago by
dhruv pant30
dhruv pant30 wrote:
Rafael A. Irizarry <ririzarr at="" ...=""> writes: > > Hi! I just wanted to point out that the oligo package supports nimblegen > arrays (you need the xys and ndf) files. It is under development but we > need testers. If anybody wants a specific method or function > incorporated you can email benitlon or me. Most functions in the affy > package can be easily exproted. > > -r > Hi Rafael, I was wondering if the affyPLM function fitPLM (which takes an affybatch object) could be applied to Nimblegen ftr(xys) files. I have the ndf file and using the library makePlatformDesign, I've created the appropriate package. The oligo function read.xysfiles creates an ExpressionFeatureSet object which is incompatible for fitPLM. I've tried to convert the ftr files to Affy CEL file format, in which case I could use function ReadAffy to create an AffyBatch object. but presumably the NDF file also has to converted to CDF format?? Thanks Dhruv
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