Question: beadarray bead-level normalisation for single channel data
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gravatar for Asta Laiho
12.0 years ago by
Asta Laiho70
Asta Laiho70 wrote:
I have been using beadarray v. 1.5.10 to analyse bead-level Illumina data. At the moment the package does not support bead-level normalisation for single channel data. As I would be very interested in getting data quantile-normalised at the bead-level, before summarising, I would like to know if there are plans in the near future to add this feature in to the package. Greetings, Asta Laiho
beadarray • 432 views
ADD COMMENTlink modified 12.0 years ago by Oosting, J. PATH550 • written 12.0 years ago by Asta Laiho70
Answer: beadarray bead-level normalisation for single channel data
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gravatar for Oosting, J. PATH
12.0 years ago by
Oosting, J. PATH550 wrote:
I have also thought about between array quantile normalization for bead arrays, but the implementation is not straightforward because the arrays have different numbers of (reported) beads. For quantile normalization it will probably be necessary to pad the arrays with smaller amounts of beads, where you would have to make a decision on what the values should be in the padded data. (ie. Pad the tails with the minimum and maximum values within the array, take a sample from the existing data, or sort the data and take datapoints at regular intervals) Jan Oosting > bounces at stat.math.ethz.ch] On Behalf Of Asta Laiho > I have been using beadarray v. 1.5.10 to analyse bead-level Illumina data. > At the moment the package does not support bead-level normalisation for > single channel data. As I would be very interested in getting data > quantile-normalised > at the bead-level, before summarising, I would like to know if there are > plans in the near > future to add this feature in to the package. > > Greetings, > Asta Laiho
ADD COMMENTlink written 12.0 years ago by Oosting, J. PATH550
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