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Dear Abhilash, although it would be possible to build a microarray image analysis application from the tools provided in the EBImage package, it seems that most likely it would be an unwise investment of your time. There are many well-tested applications out there for just this task, see e.g. here for an overview: http://ihome.cuhk.edu.hk/~b400559/arraysoft_image.html and I am sure a bit of Googling will bring you more. Best wishes Wolfgang ------------------------------------------------------------------ Wolfgang Huber EBI/EMBL Cambridge UK http://www.ebi.ac.uk/huber Abhilash Venu scripsit 19/03/2008 07:17: > Dear Dr. Oleg, > > As per your direction I tried EBImage in a system which has a memory of 2GB. > (windows as operating system). Currently I am trying to extract the features > from a Agilent whole genome 4x44K chip, but when I tried to load a tif file > it has shown the following error message "cannot allocate vector of size > 245.1 Mb". Could you recommend a method to rectify this problem? > > Regards, > Abhilash > > On Wed, Feb 27, 2008 at 7:50 PM, Oleg Sklyar <osklyar at="" ebi.ac.uk=""> wrote: > >> EBImage of Bioconductor is designed for image analysis and it should be >> straight forward to apply the functions to microarray images. Best, Oleg >> >> Abhilash Venu wrote: >>> Dear all, >>> >>> I would like to know whether any package is available for micrarray >> image >>> analysis in Bioconductor and Cran. >>> >> -- >> Dr Oleg Sklyar * EBI-EMBL, Cambridge CB10 1SD, UK * +44-1223-494466 >> > > >