RE: mva.pairs
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@stephen-henderson-71
Last seen 7.7 years ago
OK try something like >Sel<-c(1,4,5,6) or >Sel<- 1:3 then >mva.pairs(exprs(eset[,Sel])) REM if you are using rma or similar to put in log.it=F (so the results are not logged again). e.g. >mva.pairs(exprs(eset[,Sel]), log.it=F) If there's a wrong un in there you'll see aline of skewed or highly spread scatters. -----Original Message----- From: Anna Gustafsson [mailto:annag@biotech.kth.se] Sent: Thursday, August 14, 2003 2:26 PM To: Stephen Henderson Subject: mva.pairs OK! Seems to generate plots. But how can I decide which chips to be compared to each other? I have 7 chips in this particular test experiment and want to be able to test each against all others more or less. Is it "doable" :)? Thanks for helping out! // Anna :o) At 14:14 2003-08-14 +0100, you wrote: >Try >>mva.pairs(exprs(eset)) > >-----Original Message----- >From: Anna Gustafsson [mailto:annag@biotech.kth.se] >Sent: Thursday, August 14, 2003 2:13 PM >To: bioconductor@stat.math.ethz.ch >Subject: [BioC] (no subject) > >Dear all, > >Unfortunately - more questions: >I wonder how to use mva.pairs to plot 2 replicate slides from an "eset" >object of background corrected, normalized, pm corrected and summarized data >(i.e rma output). > >I can only find a vignette describing plots from only normalized, non >summarized (16 individual expression measures / gene plotted) and I do not >understand how to transfer that information to work for my data in the >"eset" format..... :( > >With hopes on help! > >// Anna :o) > >********************************************************************* ****** * >*************** >Anna Gustafsson > >Royal Institute of Technology >AlbaNova University Center >Stockholm Center for Physics, Astronomy and Biotechnology >Department of Molecular Biotechnology >106 91 Stockholm, Sweden > >Phone (office): +46 8 553 783 41 >Fax: + 46 8 553 784 81 >Visiting adress: Roslagstullsbacken 21, Floor 3 >Delivery adress: Roslagsv?gen 30B > >_______________________________________________ >Bioconductor mailing list >Bioconductor@stat.math.ethz.ch >https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > >********************************************************************* * >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you have received this email in error please notify >the system manager (wibr.mail@ucl.ac.uk). All files are scanned for viruses. >********************************************************************* * ********************************************************************** ****** *************** Anna Gustafsson Royal Institute of Technology AlbaNova University Center Stockholm Center for Physics, Astronomy and Biotechnology Department of Molecular Biotechnology 106 91 Stockholm, Sweden Phone (office): +46 8 553 783 41 Fax: + 46 8 553 784 81 Visiting adress: Roslagstullsbacken 21, Floor 3 Delivery adress: Roslagsv?gen 30B ********************************************************************** ****** *************** ********************************************************************** This email and any files transmitted with it are confidentia...{{dropped}}
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Entering edit mode
@stephen-henderson-71
Last seen 7.7 years ago
ps to make it quicker you may also wish to only sample some genes (not 22,000) Try >samp<- sample(1:22000, 1000, replace=F) then >mva.pairs(exprs(eset[samp,Sel]), log.it=F) -----Original Message----- From: Anna Gustafsson [mailto:annag@biotech.kth.se] Sent: Thursday, August 14, 2003 2:26 PM To: Stephen Henderson Subject: mva.pairs OK! Seems to generate plots. But how can I decide which chips to be compared to each other? I have 7 chips in this particular test experiment and want to be able to test each against all others more or less. Is it "doable" :)? Thanks for helping out! // Anna :o) At 14:14 2003-08-14 +0100, you wrote: >Try >>mva.pairs(exprs(eset)) > >-----Original Message----- >From: Anna Gustafsson [mailto:annag@biotech.kth.se] >Sent: Thursday, August 14, 2003 2:13 PM >To: bioconductor@stat.math.ethz.ch >Subject: [BioC] (no subject) > >Dear all, > >Unfortunately - more questions: >I wonder how to use mva.pairs to plot 2 replicate slides from an "eset" >object of background corrected, normalized, pm corrected and summarized data >(i.e rma output). > >I can only find a vignette describing plots from only normalized, non >summarized (16 individual expression measures / gene plotted) and I do not >understand how to transfer that information to work for my data in the >"eset" format..... :( > >With hopes on help! > >// Anna :o) > >********************************************************************* ****** * >*************** >Anna Gustafsson > >Royal Institute of Technology >AlbaNova University Center >Stockholm Center for Physics, Astronomy and Biotechnology >Department of Molecular Biotechnology >106 91 Stockholm, Sweden > >Phone (office): +46 8 553 783 41 >Fax: + 46 8 553 784 81 >Visiting adress: Roslagstullsbacken 21, Floor 3 >Delivery adress: Roslagsv?gen 30B > >_______________________________________________ >Bioconductor mailing list >Bioconductor@stat.math.ethz.ch >https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > >********************************************************************* * >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you have received this email in error please notify >the system manager (wibr.mail@ucl.ac.uk). All files are scanned for viruses. >********************************************************************* * ********************************************************************** ****** *************** Anna Gustafsson Royal Institute of Technology AlbaNova University Center Stockholm Center for Physics, Astronomy and Biotechnology Department of Molecular Biotechnology 106 91 Stockholm, Sweden Phone (office): +46 8 553 783 41 Fax: + 46 8 553 784 81 Visiting adress: Roslagstullsbacken 21, Floor 3 Delivery adress: Roslagsv?gen 30B ********************************************************************** ****** *************** ********************************************************************** This email and any files transmitted with it are confidentia...{{dropped}}
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