Question about GOstatsHyperG
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affy snp ▴ 640
@affy-snp-2480
Last seen 9.7 years ago
Dear all, Hi! I have a question about using the function 'hyperGTest' in GOstatsHyperG. What if I want to find the enriched GO terms based on a set of genes which I only know gene symbol, Ensembl Gene ID, and RefSeq ID. Since the array is customized and I don't find the corresponding 'annotation' package in Bioconductor. In other words, when making the: params <- new(...,geneIds, annotation,...), would it possible for me to pass "gene symbol" or "Ensembl Gene ID", or "RefSeq ID" to "geneIds" and ignore "annotation" etc.? Thanks much! Allen [[alternative HTML version deleted]]
Annotation GO Annotation GO • 864 views
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Marc Carlson ★ 7.2k
@marc-carlson-2264
Last seen 7.8 years ago
United States
Hi Allen, In that case you can just use the RefSeq IDs to make a custom package for your platform of choice using the SQLForge code inside of the AnnotationDbi package. You can find out more here: http://www.bioconductor.org/packages/2.3/bioc/html/AnnotationDbi.html Let me know if you need more information, Marc ss wrote: > Dear all, > > Hi! I have a question about using the function 'hyperGTest' in > GOstatsHyperG. > What if I want to find the enriched GO terms based on a set of genes which I > only know gene symbol, Ensembl Gene ID, and RefSeq ID. Since the array > is customized and I don't find the corresponding 'annotation' package in > Bioconductor. > > In other words, when making the: > > params <- new(...,geneIds, annotation,...), would it possible for me to pass > "gene symbol" > or "Ensembl Gene ID", or "RefSeq ID" to "geneIds" and ignore "annotation" > etc.? > > Thanks much! > > Allen > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > >
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Thank you, Marc! Best, Allen On Thu, Jul 10, 2008 at 11:23 AM, Marc Carlson <mcarlson@fhcrc.org> wrote: > Hi Allen, > > In that case you can just use the RefSeq IDs to make a custom package for > your platform of choice using the SQLForge code inside of the AnnotationDbi > package. > You can find out more here: > > http://www.bioconductor.org/packages/2.3/bioc/html/AnnotationDbi.html > > Let me know if you need more information, > > > Marc > > > > ss wrote: > >> Dear all, >> >> Hi! I have a question about using the function 'hyperGTest' in >> GOstatsHyperG. >> What if I want to find the enriched GO terms based on a set of genes which >> I >> only know gene symbol, Ensembl Gene ID, and RefSeq ID. Since the array >> is customized and I don't find the corresponding 'annotation' package in >> Bioconductor. >> >> In other words, when making the: >> >> params <- new(...,geneIds, annotation,...), would it possible for me to >> pass >> "gene symbol" >> or "Ensembl Gene ID", or "RefSeq ID" to "geneIds" and ignore "annotation" >> etc.? >> >> Thanks much! >> >> Allen >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor@stat.math.ethz.ch >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> >> >> > > [[alternative HTML version deleted]]
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@sean-davis-490
Last seen 4 months ago
United States
On Thu, Jul 10, 2008 at 11:05 AM, ss <affysnp at="" gmail.com=""> wrote: > Dear all, > > Hi! I have a question about using the function 'hyperGTest' in > GOstatsHyperG. > What if I want to find the enriched GO terms based on a set of genes which I > only know gene symbol, Ensembl Gene ID, and RefSeq ID. Since the array > is customized and I don't find the corresponding 'annotation' package in > Bioconductor. > > In other words, when making the: > > params <- new(...,geneIds, annotation,...), would it possible for me to pass > "gene symbol" > or "Ensembl Gene ID", or "RefSeq ID" to "geneIds" and ignore "annotation" > etc.? You can use the org.hs-type annotation package, which uses entrez gene id as the key. So, you would need to get the Entrez Gene ID for the genes on your chip. Given that you have RefSeq, Ensembl, and Symbol, you can use the same annotation package to get back to Entrez Gene ID from those other ID types. Sean
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Thank you, Sean! Best, Allen On Thu, Jul 10, 2008 at 11:24 AM, Sean Davis <sdavis2@mail.nih.gov> wrote: > On Thu, Jul 10, 2008 at 11:05 AM, ss <affysnp@gmail.com> wrote: > > Dear all, > > > > Hi! I have a question about using the function 'hyperGTest' in > > GOstatsHyperG. > > What if I want to find the enriched GO terms based on a set of genes > which I > > only know gene symbol, Ensembl Gene ID, and RefSeq ID. Since the array > > is customized and I don't find the corresponding 'annotation' package in > > Bioconductor. > > > > In other words, when making the: > > > > params <- new(...,geneIds, annotation,...), would it possible for me to > pass > > "gene symbol" > > or "Ensembl Gene ID", or "RefSeq ID" to "geneIds" and ignore "annotation" > > etc.? > > You can use the org.hs-type annotation package, which uses entrez gene > id as the key. So, you would need to get the Entrez Gene ID for the > genes on your chip. Given that you have RefSeq, Ensembl, and Symbol, > you can use the same annotation package to get back to Entrez Gene ID > from those other ID types. > > Sean > [[alternative HTML version deleted]]
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