PDNN for affy.
1
0
Entering edit mode
@stephen-nyangoma-366
Last seen 10.2 years ago
Hi all, I am implementing the R for PDNN model by Laurent in R-1.7.1. The function find.params.pdnn works ok and I can obtain the Bs, Ns, Fs and the lambdas. I want to use equation 1 in the paper Li Zhang, Michael F. Miles and Kenneth D. Aldape - A model of molecular interactions on short oligonucleotide arrays, 2003, Nature Biotech., vol. 21, n.7 to obtain the fitted values. At the moment, I cannot see how to obtain the Es (especially the E^*, the average free energy for NSB). Does anyone have an idea of how to generate these values? Regards. Stephen.
• 975 views
ADD COMMENT
0
Entering edit mode
Laurent Gautier ★ 2.3k
@laurent-gautier-29
Last seen 10.2 years ago
Hi Stephen, Note that the pack 'affypdnn' was developped under R-1.7.1, but it should work out-of-the-box with R-1.8.0 and/or the latest devel version of 'affy'. I shall see if we can cram a short moment to include a function to do that before the end of the week... L. On Mon, Oct 13, 2003 at 10:01:55AM +0200, Stephen Nyangoma wrote: > Hi all, > I am implementing the R for PDNN model by Laurent in R-1.7.1. The > function find.params.pdnn works ok and I can obtain the Bs, Ns, Fs and > the lambdas. I want to use equation 1 in the paper > > Li Zhang, Michael F. Miles and Kenneth D. Aldape - > A model of molecular interactions on short oligonucleotide > arrays, 2003, Nature Biotech., vol. 21, n.7 > > to obtain the fitted values. At the moment, I cannot see how to obtain > the Es (especially the E^*, the average free energy for NSB). Does > anyone have an idea of how to generate these values? > > Regards. Stephen. > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor -- -------------------------------------------------------------- Laurent Gautier CBS, Building 208, DTU PhD. Student DK-2800 Lyngby,Denmark tel: +45 45 25 24 89 http://www.cbs.dtu.dk/laurent
ADD COMMENT
0
Entering edit mode
Thanks, Laurent for a quick reply. Stephen. On Mon, 2003-10-13 at 10:36, Laurent Gautier wrote: > Hi Stephen, > > Note that the pack 'affypdnn' was developped under R-1.7.1, > but it should work out-of-the-box with R-1.8.0 and/or > the latest devel version of 'affy'. > > I shall see if we can cram a short moment to include a function > to do that before the end of the week... > > L. > > > On Mon, Oct 13, 2003 at 10:01:55AM +0200, Stephen Nyangoma wrote: > > Hi all, > > I am implementing the R for PDNN model by Laurent in R-1.7.1. The > > function find.params.pdnn works ok and I can obtain the Bs, Ns, Fs and > > the lambdas. I want to use equation 1 in the paper > > > > Li Zhang, Michael F. Miles and Kenneth D. Aldape - > > A model of molecular interactions on short oligonucleotide > > arrays, 2003, Nature Biotech., vol. 21, n.7 > > > > to obtain the fitted values. At the moment, I cannot see how to obtain > > the Es (especially the E^*, the average free energy for NSB). Does > > anyone have an idea of how to generate these values? > > > > Regards. Stephen. > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > -- > -------------------------------------------------------------- > Laurent Gautier CBS, Building 208, DTU > PhD. Student DK-2800 Lyngby,Denmark > tel: +45 45 25 24 89 http://www.cbs.dtu.dk/laurent
ADD REPLY
0
Entering edit mode
Dear all, I have a problem with Bioconductor packages, and in particular affy, after updating R to 1.8.0 on a Linux\Debian box I reinstalled all Bioconductor packages but I get this errors: >library(affy) Error in bindingIsLocked(m2,where): not an environment (repeated twice) Error in getClass(Class, where=topenv(parent.frame())): "MIAME" is not a defined class Error in library(affy) : .First.lib failed Any suggestion? TIA, Ste -- Stefano Calza, Sezione di Statistica Medica Dip. di Scienze Biomediche e Biotecnologie Universit? degli Studi di Brescia - Italy Viale Europa, 11 25123 Brescia email: calza@med.unibs.it Telefono/Phone: +390303717532 Fax: +390303701157
ADD REPLY

Login before adding your answer.

Traffic: 605 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6