Question: vsn add-on normalization
gravatar for Markus Schmidberger
10.5 years ago by
Markus Schmidberger380 wrote:
Hi, I have a question for the vsn normalization towards a reference data set. (my real data are much more) I will normalize 5 Arrays with vsn: ab_ref <- ReadAffy(filenames=celfiles[1:5]) fit_ref <- vsn2(ab_ref) After this I can add 2 Arrays with normalization towards a reference: ab_add <- ReadAffy(filenames=celfiles[6:7]) eSet1 <- vsnrma(ab_add, reference=fit_ref) And than I would like to add two more Arrays: ab_add2 <- ReadAffy(filenames=celfiles[7:8]) eSet2 <- vsnrma(ab_add2, reference=fit_ref) Do I use the old reference (fit_ref) for the second add-on normalization or the fit from the first add-on normalization or a combination of the first two fits? Thanks Markus -- Dipl.-Tech. Math. Markus Schmidberger Ludwig-Maximilians-Universit?t M?nchen IBE - Institut f?r medizinische Informationsverarbeitung, Biometrie und Epidemiologie Marchioninistr. 15, D-81377 Muenchen URL: Mail: Markus.Schmidberger [at] Tel: +49 (089) 7095 - 4497
normalization vsn • 505 views
ADD COMMENTlink written 10.5 years ago by Markus Schmidberger380
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 291 users visited in the last hour