Hallo everybody, I'm following the commands > library(flowQ) > data(GvHD) > a<-GvHD[1:3] > dest<- file.path(tempdir(), "flowQ")* > qp1<- qaProcess.cellnumber(a, outdir = dest, cFactor = 0.75, pdf = T) > qp2<- qaProcess.marginevents(a, channels = c("FSC-H", "SSC-H"), outdir = dest) > qp4<- qaProcess.timeflow(a, outdir = dest, cutoff = 2)* and get error message: creating summary plots...Error en if (scaleFac != 1) newDims<- dims * scaleFac : valor ausente donde TRUE/FALSE es necesario Además: Mensajes de aviso perdidos In .local(.Object, ...) : NAs introducidos por coerción (I would translate as: missing value where TRUE/FALSE needed more: alarm messages lost In .local(.Object, ...) : NAs inserted by force) and using *> qp3<- qaProcess.timeline(a, channels = "FSC-H", outdir = dest, cutoff = 1) creating summary plots....Par metro no v lido: 240x240* Error en .local(.Object, ...) : Unable to find file 'C:/DOCUME~1/ewelina/CONFIG~1/Temp/Rtmpl7aftl/f lowQ/images/jgEtkNPVuT/summary.pdf' Además: Mensajes de aviso perdidos In sysFun(paste("convert ", " -density 240x240 +append ", paste(sfiles, : 'convert -density 240x240 +append C:/DOCUME~1/ewelina/CONFIG~1/Tem p/Rtmpl7aftl/flowQ/images/jgEtkNPVuT/summary_1.pdf C:/DOCUME~1/ewelina /CONFIG~1/Temp/Rtmpl7aftl/flowQ/images/jgEtkNPVuT/summary.pdf' execution failed with error code 4 I can successfully follow the commands above in Fedora with R 2.10.0 and flowQ_1.6.0 installed but not in windows: > > sessionInfo() > R version 2.11.1 (2010-05-31) i386-pc-mingw32 locale: [1] LC_COLLATE=Spanish_Spain.1252 LC_CTYPE=Spanish_Spain.1252 [3] LC_MONETARY=Spanish_Spain.1252 LC_NUMERIC=C [5] LC_TIME=Spanish_Spain.1252 attached base packages: [1] tools stats graphics grDevices utils datasets methods [8] base other attached packages: [1] flowQ_1.8.0 latticeExtra_0.6-11 RColorBrewer_1.0-2 [4] parody_1.6.0 bioDist_1.20.0 KernSmooth_2.23-3 [7] mvoutlier_1.4 flowViz_1.12.0 flowCore_1.14.1 [10] rrcov_1.0-01 pcaPP_1.8-1 mvtnorm_0.9-9 [13] robustbase_0.5-0-1 Biobase_2.8.0 lattice_0.18-8 [16] outliers_0.13-2 loaded via a namespace (and not attached): [1] annotate_1.26.0 AnnotationDbi_1.10.1 DBI_0.2-5 [4] feature_1.2.4 geneplotter_1.26.0 graph_1.26.0 [7] grid_2.11.1 ks_1.6.13 MASS_7.3-6 [10] RSQLite_0.9-1 stats4_2.11.1 xtable_1.5-6 thanks in advance! best wishes, Ewelina Bakala [[alternative HTML version deleted]]