Hi, I am a statistician at the Princess Margaret Hospital and I will need to do some analysis on data from microarray. I have taken some courses, among them, being the course given by Terry Speed in which, the Bioconductor packages were introduced. Our UNIX administrator have downloaded R and the Bioconductor packages and installed them on our UNIX machine. However I have several problems when I try to use them. I would appreciate if you could shed some light and give me a hint on what should be done. Here are the problems which I encountered: 1) when I try to load the library marrayNorm: among the notes which come up on the screen there is: Error in dyn.load(x, as.logical(local), as.logical(now)) : unable to load shared library "/usr/local/R-1.5.1/library/tcltk/libs/tcltk.so": ld.so.1: /usr/local/R-1.5.1/bin/R.bin: fatal: relocation error: file /usr/local/R-1.5.1/ library/tcltk/libs/tcltk.so: symbol Tcl_GetString: referenced symbol not found Error in .C("delTcl", PACKAGE = "tcltk") : C/Fortran function name not in load table and also: Warning message: There is no package called `marrayPlots' in: library(package, char = TRUE, logical = TRUE, warn.conflicts = warn.conflicts, 2) after quitting R with the option to save the work space image and re-starting R, I cannot use the functions in the libraries which were previously loaded (for example the function maNorm, which was for sure loaded with its help file). Do I have to load the libraries every time I am starting R? 3) The library sma doesn't seem to be in the package bundle either. Thank you. Melania Melania Pintilie Department of Biostatistics, Rm. 15-433 Ontario Cancer Institute Princess Margaret Hospital 610 University Ave. Toronto, ON, M5G 2M9 Tel: (416) 946-4501 ext. 4886 Fax: (416) 946-2048

> Error in dyn.load(x, as.logical(local), as.logical(now)) : > unable to load shared library > "/usr/local/R-1.5.1/library/tcltk/libs/tcltk.so": > ld.so.1: /usr/local/R-1.5.1/bin/R.bin: fatal: relocation error: > file /usr/local/R-1.5.1/ > library/tcltk/libs/tcltk.so: symbol Tcl_GetString: referenced symbol > not found Do you have package 'tcltk' installed? If not, that needs to be installed. If so, is Tcl/TK (the libraries, not the R package) installed on the system? > 3) The library sma doesn't seem to be in the package bundle either. Its not, as it isn't a Bioconductor package. -J

> 2) after quitting R with the option to save the work space > image and > re-starting R, I cannot use the functions in the libraries which were > previously loaded (for example the function maNorm, which was > for sure > loaded with its help file). > Do I have to load the libraries every time I am starting R? > Yes, you do have to reload the libraries each time. -Greg LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.