Hello: I have faced a problem when I´m trying to analyze my TLDA data: I have 3 or 2 technical replicates per sample, my problem is that I really don´t know how to read and asses the replicates. What I tried is to analyze my data as individual samples and then asses the replicates as following: d.normA2= exprs(d.normA) > d.normA2.prom= (d.normA2[,1]+ d.normA[,2]+ d.normA2[,3])/3) > d.normA2.prom= cbind(d.normA2.prom,(d.normA2[,4] + d.normA2[,5]+ d.normA2[,6])/3) > d.normA2.prom= cbind(d.normA2.prom,(d.normA2[,11] + d.normA2[,12]) + d.normaA2[,13])/3) But I get: Error: inesperado ')' en "d.normA2.prom= (d.normA2[,1]+ d.normA[,2]+ d.normA2[,3])/3)" I don´t know which is the best option to read my replicates because I´m trying to evaluate 74 TLDA files corresponding to 30 samples, with technical replicates. Thank you a lot. Biol. Sandra Lorena Romero Córdoba Ph student at Autonomous National University of Mexico Lab. Genomics of Cancer, National Institute of Genomic Medicine [[alternative HTML version deleted]]