RmiR: mapping miRNA to probe_id for Affy mogene10st
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Mary Putt ▴ 100
@mary-putt-3120
Last seen 9.6 years ago
Hi I have data from mouse and would like to map miRNA to probeset iDs. I have used RmiR and get an error msg that I need to install RmiR.MUS.miRNA' but this annotation package does not exist. Have also ignored the org argument but this also yields an error. New to this so apologies if this is a dumb ? My code follows Many thanks in advance, Mary Putt > mirnas<-data.frame(siggenes$Gene.ID) > mirnas$expr<-siggenes$Score.d. > > > genes<-data.frame(siggenes.mrna$Gene.Name) > genes$expr<-siggenes.mrna$Score.d. > > names(genes)<-c("genes", "expr") > names(mirnas)<-c("mirna", "expr") > > dim(genes) [1] 87 2 > dim(mirnas) [1] 10 2 > genes[1:5,] genes expr 1 10386996 -9.307098 2 10594609 -9.314124 3 10578017 -7.941403 4 10479625 -8.024112 5 10385995 -8.058759 > mirnas[1:5,] mirna expr 1 mmu-let-7d-star_st -3.53168874110412 2 mmu-miR-297a_st -4.60638517072762 3 mmu-miR-678_st -3.4425261537353 4 mmu-miR-183-star_st -3.57272865665287 5 mmu-miR-223_st -3.86083950583795 > ############ #specify MUS ############## > read.mir(genes = genes, mirna = mirnas, annotation = "mogene10sttranscriptcluster.db", id="probes", org="MUS") Loading required package: RmiR.MUS.miRNA Error in RmiR(genes = genes, mirna = mirna, annotation = annotation, id = id, : RmiR.MUS.miRNA package must be installed first In addition: Warning message: In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, : there is no package called 'RmiR.MUS.miRNA' > > ############## #ignore org ################## >read.mir(genes = genes, mirna = mirnas, annotation = "mogene10sttranscriptcluster.db", id="probes") Error in `$<-.data.frame`(`*tmp*`, "mirCV", value = NA) : replacement has 1 rows, data has 0
miRNA Annotation RmiR miRNA Annotation RmiR • 824 views
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