Hi, Annette On 2011-01-21, at 7:55 AM, Annette M Hynes wrote: > Thanks, Wolfgang. > > I have looked at the different flow cytometry packages, but I haven't found anything for deconvoluting DNA histograms. Are you referring to 1D mixtures? The flowClust package should easily be up to the task (http://bioconductor.org/help/bioc- views/2.8/bioc/html/flowClust.html), have you tried that? Even if your data is multidimensional, flowClust is designed expressly for fitting multidimensional mixtures. > This might be because I'm also getting blanks for many of the "reference manuals." Have I missed something? Forgive me for asking a naive question, but what are the "reference manuals" in this context? Cheers, Greg Finak, PhD Post-doctoral Research Associate PS Statistics, Vaccine and Infectious Disease Division. Fred Hutchinson Cancer Research Center Seattle, WA (206)667-3116 gfinak at fhcrc.org > > Annette > > --------------------- > Annette M. Hynes, PhD > Postdoctoral Research Associate > University of Georgia > Department of Marine Sciences > 247 Marine Science Building > Athens, GA 30602-3636 > tel: 706-542-2405 > > > > ------------------------------ > > Message: 13 > Date: Fri, 21 Jan 2011 01:44:53 +0100 > From: Wolfgang Huber <whuber at="" embl.de=""> > To: bioconductor at r-project.org > Subject: Re: [BioC] Cell cycle analysis > Message-ID: <4D38D705.6040801 at embl.de> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > Annette, > > besides the basic R functions for thresholding, clustering, mixture > modelling, have you looked at the flow* packages already? - > http://www.bioconductor.org/help/workflows/high-throughput-assays > > Best wishes > Wolfgang > > Il Jan/20/11 5:57 PM, Annette M Hynes ha scritto: >> Greetings. >> >> I was wondering if anyone knew of an R package for cell cycle analysis. I would like to deconvolve DNA histograms from flow cytometry data into S, G1, and G2 phases. Someone had asked this same question 5 years ago, and I was hoping there might have been some developments. >> >> Thanks, >> >> Annette >> >> --------------------- >> Annette M. Hynes, PhD >> Postdoctoral Research Associate >> University of Georgia >> Department of Marine Sciences >> 247 Marine Science Building >> Athens, GA 30602-3636 >> tel: 706-542-2405 >> >> [[alternative HTML version deleted]] >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > -- > > > Wolfgang Huber > EMBL > http://www.embl.de/research/units/genome_biology/huber > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor