Hi, I am using RNA-seq data to identify the genes that are differentially expressed. I have two groups (eg., F0 and P6) with two replicates (a and b) for each group for the analysis. I was little confused which analysis to use, either "Analysis using common dispersion" OR "Analysis using moderated tagwise dispersions". I was not sure as to what value to use for prior.n as I have two groups with two replicates each. I would appreciate if you could help me in deciding the method for analysis. Thanks, Sridhara -- Sridhara G Kunjeti PhD Candidate University of Delaware Department of Plant and Soil Science email- sridhara@udel.edu Ph: 832-566-0011 [[alternative HTML version deleted]]