Hello, What I would like to do seems like a common application in analysis and I am probably missing a very easy way to accomplish this. I would like to apply a 'memory gate' generated by quadrantGate to both CD4 and CD8 lymphocyte populations. However, there appears to be no way to give the second gate a unique name for the workFlow. I use a quadrantGate to create filters for channels PE.Cy7.A and PerCP.Cy5.5.A. The quadrantGates are slightly different for the CD4 and CD8 populations (though that does not matter here). The 'action_' name in the workFlow is different for each one, but the workFlow node name appears to be the same thus there is no easy way to call the population for a plot. After adding the gate I must use an arbitrary gateview ID for plotting, which is not at all convenient especially with Sweave. Is there a way around this? Below is a short example. Thanks, Aric ----------------- quadGate.memory.CD4 <- quadrantGate(Data(wf[['CD4+CD8-']]), c("PerCP.Cy5.5.A","PE.Cy7.A"), plot=TRUE, alpha=c(0.5, 1), filterId="CD4_MEMORY" ) add(wf, quadGate.memory.CD4, parent="CD4+CD8-") quadGate.memory.CD8.base <- quadrantGate(Data(wf[['CD4-CD8+']])[[5]], c("PerCP.Cy5.5.A","PE.Cy7.A"), plot=TRUE, alpha=c(0.5, 0.9), filterId="CD8_MEMORY" ) add(wf, quadGate.memory.CD8.base, parent="CD4-CD8+") Warning: 'CD45ra+CCR7+' is not a unique view name in this workFlow object. You will only be able to access the view using its UID 'gateViewRef_OkTFWgIFTF'

On Mon, 14 Nov 2011 15:58:09 -0800 Aric Gregson <a.gregson at="" ucla.edu=""> wrote: > Is there a way around this? OK. I have come up with a way around this, although considering a more global answer for working with the workFlow and perhaps a different naming scheme for nodes/gateViews may be better in the long run. I can plot the gate using the filter results without adding it to the workFlow: > memory.cd8.quadgate.base.result <- filter(Data(wf[['CD4-CD8+']]), quadGate.memory.CD8.base) > xyplot(`PE.Cy7.A` ~ `PerCP.Cy5.5.A` | SampleID, Data(wf[['CD4-CD8+']]), filter=memory.cd8.quadgate.base.result) I can extract the 'boundary' results from the quadGate result and create multiple rectangleGates to add to the workFlow, for example: > memory.cd8.naive <- rectangleGate("PerCP.Cy5.5.A"=c(quadGate.memory.CD8.base at boundary[[1]], Inf), "PE.Cy7.A"=c(quadGate.memory.CD8.base at boundary[[2]], Inf), filterId="CD8_NAIVE")