arrayQualityMetrics produces non-interactive PCA plot (Figure 3)
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@vladimir-zhurov-4990
Last seen 10.3 years ago
> This is the first post, so I am not top-posting. Hello all, I am having a problem with the arrayQualityMetrics interactive SVG graphics. The system is Ubuntu 10.04.3 LTS. Viewing was done in Firefox 8 and Chrome 15. I would greatly appreciate any help with this issue. Below is the information about system, libraries version and sample R session. $ uname -a Linux 2.6.32-36-generic #79-Ubuntu SMP Tue Nov 8 22:29:53 UTC 2011 x86_64 GNU/Linux $ pkg-config --modversion cairo libxml-2.0 1.10.2 2.7.8 $ R --vanilla R version 2.14.0 (2011-10-31) Copyright (C) 2011 The R Foundation for Statistical Computing ISBN 3-900051-07-0 Platform: x86_64-pc-linux-gnu (64-bit) R is free software and comes with ABSOLUTELY NO WARRANTY. You are welcome to redistribute it under certain conditions. Type 'license()' or 'licence()' for distribution details. Natural language support but running in an English locale R is a collaborative project with many contributors. Type 'contributors()' for more information and 'citation()' on how to cite R or R packages in publications. Type 'demo()' for some demos, 'help()' for on-line help, or 'help.start()' for an HTML browser interface to help. Type 'q()' to quit R. > library("ALLMLL") Loading required package: affy Loading required package: Biobase Welcome to Bioconductor Vignettes contain introductory material. To view, type 'browseVignettes()'. To cite Bioconductor, see 'citation("Biobase")' and for packages 'citation("pkgname")'. > data("MLL.A") > library("arrayQualityMetrics") > arrayQualityMetrics(MLL.A, + outdir="MLL.A_Report", + force=TRUE, + do.logtransform=TRUE) The directory 'MLL.A_Report' has been created. Loading required package: AnnotationDbi KernSmooth 2.23 loaded Copyright M. P. Wand 1997-2009 > traceback() No traceback available > warnings() NULL > sessionInfo() R version 2.14.0 (2011-10-31) Platform: x86_64-pc-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8 [5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8 [7] LC_PAPER=C LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] hgu133acdf_2.9.1 AnnotationDbi_1.16.8 [3] arrayQualityMetrics_3.10.0 ALLMLL_1.2.11 [5] affy_1.32.0 Biobase_2.14.0 loaded via a namespace (and not attached): [1] affyio_1.22.0 affyPLM_1.30.0 annotate_1.32.0 [4] beadarray_2.4.1 BiocInstaller_1.2.1 Biostrings_2.22.0 [7] Cairo_1.5-0 cluster_1.14.1 DBI_0.2-5 [10] genefilter_1.36.0 grid_2.14.0 Hmisc_3.9-0 [13] hwriter_1.3 IRanges_1.12.4 KernSmooth_2.23-7 [16] lattice_0.20-0 latticeExtra_0.6-19 limma_3.10.0 [19] preprocessCore_1.16.0 RColorBrewer_1.0-5 RSQLite_0.11.0 [22] setRNG_2009.11-1 splines_2.14.0 survival_2.36-10 [25] SVGAnnotation_0.9-0 tools_2.14.0 vsn_3.22.0 [28] XML_3.6-1 xtable_1.6-0 zlibbioc_1.0.0 More details that I had to move down here due to "top-posting" error from the web form interface. I apologize for the inconvenience. How is one supposed to post a R session? Only figure 3 is broken, figures 6 and 12 are interactive. I have attempted previously discussed fixes such as updating cairo and libxml.
arrayQualityMetrics arrayQualityMetrics • 1.5k views
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@wolfgang-huber-3550
Last seen 3 months ago
EMBL European Molecular Biology Laborat…
Dear Vladimir thank you for the precise report. This is peculiar, I just ran your example script on a very similar installation (see below), and Figure 3, the PCA plot, comes out as intended (i.e. interactive). Do you get the message "Note: the figure is static - enhancement with interactive effects failed..." ? This is what I tried: $ uname -a Darwin spinoza 11.2.0 Darwin Kernel Version 11.2.0: Tue Aug 9 20:54:00 PDT 2011; root:xnu-1699.24.8~1/RELEASE_X86_64 x86_64 $ pkg-config --modversion cairo libxml-2.0 1.10.2 2.7.8 > sessionInfo() R Under development (unstable) (2011-12-02 r57789) Platform: x86_64-apple-darwin11.2.0/x86_64 (64-bit) locale: [1] C attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] hgu133acdf_2.9.1 AnnotationDbi_1.17.9 [3] arrayQualityMetrics_3.11.0 ALLMLL_1.2.11 [5] affy_1.33.2 Biobase_2.15.2 [7] BiocGenerics_0.1.3 BiocInstaller_1.3.3 [9] fortunes_1.4-2 loaded via a namespace (and not attached): [1] Biostrings_2.23.4 Cairo_1.5-0 DBI_0.2-5 [4] Hmisc_3.9-0 IRanges_1.13.13 KernSmooth_2.23-7 [7] RColorBrewer_1.0-5 RSQLite_0.11.0 SVGAnnotation_0.9-0 [10] XML_3.6-1 affyPLM_1.31.0 affyio_1.23.1 [13] annotate_1.33.0 beadarray_2.5.2 cluster_1.14.1 [16] genefilter_1.37.0 grid_2.15.0 hwriter_1.3 [19] lattice_0.20-0 latticeExtra_0.6-19 limma_3.11.1 [22] preprocessCore_1.17.1 setRNG_2009.11-1 splines_2.15.0 [25] survival_2.36-10 tools_2.15.0 vsn_3.23.0 [28] xtable_1.6-0 zlibbioc_1.1.0 There haven't been any relevant changes from arrayQualityMetrics_3.10.0 to arrayQualityMetrics_3.11.0, so only the potentially relevant difference I can see is the locale. Can you try with "C"? Also, can you make the report that you get available, so I can see what the differences in the generated SVG are to the one that I get. This could then, potentially, be useful information for the maintainer of the SVGAnnotation package (on which arrayQualityMetrics relies) if your problem is indeed related to that. Thanks and best wishes Wolfgang On 12/3/11 2:40 AM, Vladimir Zhurov wrote: >> This is the first post, so I am not top-posting. > > Hello all, > > I am having a problem with the arrayQualityMetrics interactive SVG graphics. > The system is Ubuntu 10.04.3 LTS. Viewing was done in Firefox 8 and Chrome 15. > > I would greatly appreciate any help with this issue. > > Below is the information about system, libraries version and sample R session. > > $ uname -a > Linux 2.6.32-36-generic #79-Ubuntu SMP Tue Nov 8 22:29:53 UTC 2011 > x86_64 GNU/Linux > > $ pkg-config --modversion cairo libxml-2.0 > 1.10.2 > 2.7.8 > > $ R --vanilla > > R version 2.14.0 (2011-10-31) > Copyright (C) 2011 The R Foundation for Statistical Computing > ISBN 3-900051-07-0 > Platform: x86_64-pc-linux-gnu (64-bit) > > R is free software and comes with ABSOLUTELY NO WARRANTY. > You are welcome to redistribute it under certain conditions. > Type 'license()' or 'licence()' for distribution details. > > Natural language support but running in an English locale > > R is a collaborative project with many contributors. > Type 'contributors()' for more information and > 'citation()' on how to cite R or R packages in publications. > > Type 'demo()' for some demos, 'help()' for on-line help, or > 'help.start()' for an HTML browser interface to help. > Type 'q()' to quit R. > >> library("ALLMLL") > Loading required package: affy > Loading required package: Biobase > > Welcome to Bioconductor > > Vignettes contain introductory material. To view, type > 'browseVignettes()'. To cite Bioconductor, see > 'citation("Biobase")' and for packages 'citation("pkgname")'. > >> data("MLL.A") >> library("arrayQualityMetrics") >> arrayQualityMetrics(MLL.A, > + outdir="MLL.A_Report", > + force=TRUE, > + do.logtransform=TRUE) > The directory 'MLL.A_Report' has been created. > Loading required package: AnnotationDbi > > KernSmooth 2.23 loaded > Copyright M. P. Wand 1997-2009 >> traceback() > No traceback available >> warnings() > NULL >> sessionInfo() > R version 2.14.0 (2011-10-31) > Platform: x86_64-pc-linux-gnu (64-bit) > > locale: > [1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C > [3] LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8 > [5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8 > [7] LC_PAPER=C LC_NAME=C > [9] LC_ADDRESS=C LC_TELEPHONE=C > [11] LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] hgu133acdf_2.9.1 AnnotationDbi_1.16.8 > [3] arrayQualityMetrics_3.10.0 ALLMLL_1.2.11 > [5] affy_1.32.0 Biobase_2.14.0 > > loaded via a namespace (and not attached): > [1] affyio_1.22.0 affyPLM_1.30.0 annotate_1.32.0 > [4] beadarray_2.4.1 BiocInstaller_1.2.1 Biostrings_2.22.0 > [7] Cairo_1.5-0 cluster_1.14.1 DBI_0.2-5 > [10] genefilter_1.36.0 grid_2.14.0 Hmisc_3.9-0 > [13] hwriter_1.3 IRanges_1.12.4 KernSmooth_2.23-7 > [16] lattice_0.20-0 latticeExtra_0.6-19 limma_3.10.0 > [19] preprocessCore_1.16.0 RColorBrewer_1.0-5 RSQLite_0.11.0 > [22] setRNG_2009.11-1 splines_2.14.0 survival_2.36-10 > [25] SVGAnnotation_0.9-0 tools_2.14.0 vsn_3.22.0 > [28] XML_3.6-1 xtable_1.6-0 zlibbioc_1.0.0 > > More details that I had to move down here due to "top-posting" error from the > web form interface. > I apologize for the inconvenience. How is one supposed to post a R session? > > Only figure 3 is broken, figures 6 and 12 are interactive. I have attempted > previously discussed fixes such as updating cairo and libxml. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Best wishes Wolfgang Wolfgang Huber EMBL http://www.embl.de/research/units/genome_biology/huber
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@vladimir-zhurov-4990
Last seen 10.3 years ago
> ... Dear Wolfgang, Thank you for such a quick reply. To answer your question, yes, there was a note generated after figure 3 legend: "Note: the figure is static - enhancement with interactive effects failed..." Ultimately, I have solved the problem. It was not locale (I have tried your suggestion to set everything to "C" and it did not change the result) but XML sub-system. The thing that fixed it was the installation of the latest version of libxslt from ftp://xmlsoft.org/libxml2/ (http://xmlsoft.org/). The complete procedure (but I do not know whether _every_ step except for libxslt was necessary in fact) involved: The latest stable cairo, cairomm (required additional installation of libsigc++-2.0-dev to build on Ubuntu 10.04.3) and pixman from http://cairographics.org/ (cairo needs pixman to compile, and I added cairomm to get to the same version level). And the latest libxml-2.0 and libxslt from http://xmlsoft.org/ $ pkg-config --modversion cairo libxml-2.0 libxslt 1.10.2 2.7.8 1.1.26 Recompiled all Bioconductor packages as per instructions on the main site. source("http://bioconductor.org/biocLite.R") pkgs <- rownames(installed.packages()) biocLite(pkgs) ...and it worked. Below is the current working session info. And if you are still interested, I have broken report at: http://devbiol.zoo.uwo.ca/~vzhurov/MLL_A_Report_broken.tar.gz > sessionInfo() R version 2.14.0 (2011-10-31) Platform: x86_64-pc-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8 [5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8 [7] LC_PAPER=C LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] hgu133acdf_2.9.1 AnnotationDbi_1.16.8 [3] arrayQualityMetrics_3.10.0 ALLMLL_1.2.11 [5] affy_1.32.0 Biobase_2.14.0 loaded via a namespace (and not attached): [1] affyio_1.22.0 affyPLM_1.30.0 annotate_1.32.0 [4] beadarray_2.4.1 BiocInstaller_1.2.1 Biostrings_2.22.0 [7] Cairo_1.5-0 cluster_1.14.1 DBI_0.2-5 [10] genefilter_1.36.0 grid_2.14.0 Hmisc_3.9-0 [13] hwriter_1.3 IRanges_1.12.4 KernSmooth_2.23-7 [16] lattice_0.20-0 latticeExtra_0.6-19 limma_3.10.0 [19] preprocessCore_1.16.0 RColorBrewer_1.0-5 RSQLite_0.11.0 [22] setRNG_2009.11-1 splines_2.14.0 survival_2.36-10 [25] SVGAnnotation_0.9-0 tools_2.14.0 vsn_3.22.0 [28] XML_3.6-1 xtable_1.6-0 zlibbioc_1.0.0 Again, thank you for help and arrayQualityMetrics. Regards. Vladimir. > Wolfgang Huber <whuber at="" ...=""> writes: > > Dear Vladimir > > thank you for the precise report. This is peculiar, I just ran your > example script on a very similar installation (see below), and Figure 3, > the PCA plot, comes out as intended (i.e. interactive). > > Do you get the message "Note: the figure is static - enhancement with > interactive effects failed..." ? > > This is what I tried: > > $ uname -a > Darwin spinoza 11.2.0 Darwin Kernel Version 11.2.0: Tue Aug 9 20:54:00 > PDT 2011; root:xnu-1699.24.8~1/RELEASE_X86_64 x86_64 > > $ pkg-config --modversion cairo libxml-2.0 > 1.10.2 > 2.7.8 > > > sessionInfo() > R Under development (unstable) (2011-12-02 r57789) > Platform: x86_64-apple-darwin11.2.0/x86_64 (64-bit) > > locale: > [1] C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] hgu133acdf_2.9.1 AnnotationDbi_1.17.9 > [3] arrayQualityMetrics_3.11.0 ALLMLL_1.2.11 > [5] affy_1.33.2 Biobase_2.15.2 > [7] BiocGenerics_0.1.3 BiocInstaller_1.3.3 > [9] fortunes_1.4-2 > > loaded via a namespace (and not attached): > [1] Biostrings_2.23.4 Cairo_1.5-0 DBI_0.2-5 > [4] Hmisc_3.9-0 IRanges_1.13.13 KernSmooth_2.23-7 > [7] RColorBrewer_1.0-5 RSQLite_0.11.0 SVGAnnotation_0.9-0 > [10] XML_3.6-1 affyPLM_1.31.0 affyio_1.23.1 > [13] annotate_1.33.0 beadarray_2.5.2 cluster_1.14.1 > [16] genefilter_1.37.0 grid_2.15.0 hwriter_1.3 > [19] lattice_0.20-0 latticeExtra_0.6-19 limma_3.11.1 > [22] preprocessCore_1.17.1 setRNG_2009.11-1 splines_2.15.0 > [25] survival_2.36-10 tools_2.15.0 vsn_3.23.0 > [28] xtable_1.6-0 zlibbioc_1.1.0 > > There haven't been any relevant changes from arrayQualityMetrics_3.10.0 > to arrayQualityMetrics_3.11.0, so only the potentially relevant > difference I can see is the locale. Can you try with "C"? > > Also, can you make the report that you get available, so I can see what > the differences in the generated SVG are to the one that I get. This > could then, potentially, be useful information for the maintainer of the > SVGAnnotation package (on which arrayQualityMetrics relies) if your > problem is indeed related to that. > > Thanks and best wishes > Wolfgang > > On 12/3/11 2:40 AM, Vladimir Zhurov wrote: > >> This is the first post, so I am not top-posting. > > > > Hello all, > > > > I am having a problem with the arrayQualityMetrics interactive > > SVG graphics. > > The system is Ubuntu 10.04.3 LTS. Viewing was done in Firefox 8 and > > Chrome 15. > > > > I would greatly appreciate any help with this issue. > > > > Below is the information about system, libraries version > > and sample R session. > > > > $ uname -a > > Linux 2.6.32-36-generic #79-Ubuntu SMP Tue Nov 8 22:29:53 UTC 2011 > > x86_64 GNU/Linux > > > > $ pkg-config --modversion cairo libxml-2.0 > > 1.10.2 > > 2.7.8 > > > > $ R --vanilla > > > > R version 2.14.0 (2011-10-31) > > Copyright (C) 2011 The R Foundation for Statistical Computing > > ISBN 3-900051-07-0 > > Platform: x86_64-pc-linux-gnu (64-bit) > > > > R is free software and comes with ABSOLUTELY NO WARRANTY. > > You are welcome to redistribute it under certain conditions. > > Type 'license()' or 'licence()' for distribution details. > > > > Natural language support but running in an English locale > > > > R is a collaborative project with many contributors. > > Type 'contributors()' for more information and > > 'citation()' on how to cite R or R packages in publications. > > > > Type 'demo()' for some demos, 'help()' for on-line help, or > > 'help.start()' for an HTML browser interface to help. > > Type 'q()' to quit R. > > > >> library("ALLMLL") > > Loading required package: affy > > Loading required package: Biobase > > > > Welcome to Bioconductor > > > > Vignettes contain introductory material. To view, type > > 'browseVignettes()'. To cite Bioconductor, see > > 'citation("Biobase")' and for packages 'citation("pkgname")'. > > > >> data("MLL.A") > >> library("arrayQualityMetrics") > >> arrayQualityMetrics(MLL.A, > > + outdir="MLL.A_Report", > > + force=TRUE, > > + do.logtransform=TRUE) > > The directory 'MLL.A_Report' has been created. > > Loading required package: AnnotationDbi > > > > KernSmooth 2.23 loaded > > Copyright M. P. Wand 1997-2009 > >> traceback() > > No traceback available > >> warnings() > > NULL > >> sessionInfo() > > R version 2.14.0 (2011-10-31) > > Platform: x86_64-pc-linux-gnu (64-bit) > > > > locale: > > [1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C > > [3] LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8 > > [5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8 > > [7] LC_PAPER=C LC_NAME=C > > [9] LC_ADDRESS=C LC_TELEPHONE=C > > [11] LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C > > > > attached base packages: > > [1] stats graphics grDevices utils datasets methods base > > > > other attached packages: > > [1] hgu133acdf_2.9.1 AnnotationDbi_1.16.8 > > [3] arrayQualityMetrics_3.10.0 ALLMLL_1.2.11 > > [5] affy_1.32.0 Biobase_2.14.0 > > > > loaded via a namespace (and not attached): > > [1] affyio_1.22.0 affyPLM_1.30.0 annotate_1.32.0 > > [4] beadarray_2.4.1 BiocInstaller_1.2.1 Biostrings_2.22.0 > > [7] Cairo_1.5-0 cluster_1.14.1 DBI_0.2-5 > > [10] genefilter_1.36.0 grid_2.14.0 Hmisc_3.9-0 > > [13] hwriter_1.3 IRanges_1.12.4 KernSmooth_2.23-7 > > [16] lattice_0.20-0 latticeExtra_0.6-19 limma_3.10.0 > > [19] preprocessCore_1.16.0 RColorBrewer_1.0-5 RSQLite_0.11.0 > > [22] setRNG_2009.11-1 splines_2.14.0 survival_2.36-10 > > [25] SVGAnnotation_0.9-0 tools_2.14.0 vsn_3.22.0 > > [28] XML_3.6-1 xtable_1.6-0 zlibbioc_1.0.0 > > > > More details that I had to move down here due to "top-posting" error from > > the > > web form interface. > > I apologize for the inconvenience. > > How is one supposed to post a R session? > > > > Only figure 3 is broken, figures 6 and 12 are interactive. > > I have attempted > > previously discussed fixes such as updating cairo and libxml. > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at ... > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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Dear Vladimir thank you! I am glad it worked out. The sensitive dependency on system dependencies (and our failure to usefully diagnose when and when not they are fulfilled on a user's system) is a big concern, although I see no easy short term solution to change that. Best wishes Wolfgang On 12/5/11 6:06 AM, Vladimir Zhurov wrote: >> ... > > Dear Wolfgang, > > Thank you for such a quick reply. > > To answer your question, yes, there was a note generated after figure 3 legend: > "Note: the figure is static - enhancement with interactive effects failed..." > > Ultimately, I have solved the problem. It was not locale (I have tried your > suggestion to set everything to "C" and it did not change the result) but XML > sub-system. > > The thing that fixed it was the installation of the latest version of libxslt > from ftp://xmlsoft.org/libxml2/ (http://xmlsoft.org/). > > The complete procedure (but I do not know whether _every_ step except for > libxslt was necessary in fact) involved: > > The latest stable cairo, cairomm (required additional installation of > libsigc++-2.0-dev to build on Ubuntu 10.04.3) and pixman from > http://cairographics.org/ (cairo needs pixman to compile, and I added > cairomm to get to the same version level). > > And the latest libxml-2.0 and libxslt from http://xmlsoft.org/ > > $ pkg-config --modversion cairo libxml-2.0 libxslt > 1.10.2 > 2.7.8 > 1.1.26 > > Recompiled all Bioconductor packages as per instructions on the main site. > > source("http://bioconductor.org/biocLite.R") > pkgs<- rownames(installed.packages()) > biocLite(pkgs) > > ...and it worked. > > Below is the current working session info. > And if you are still interested, I have broken report at: > > http://devbiol.zoo.uwo.ca/~vzhurov/MLL_A_Report_broken.tar.gz > >> sessionInfo() > > R version 2.14.0 (2011-10-31) > Platform: x86_64-pc-linux-gnu (64-bit) > > locale: > [1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C > [3] LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8 > [5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8 > [7] LC_PAPER=C LC_NAME=C > [9] LC_ADDRESS=C LC_TELEPHONE=C > [11] LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] hgu133acdf_2.9.1 AnnotationDbi_1.16.8 > [3] arrayQualityMetrics_3.10.0 ALLMLL_1.2.11 > [5] affy_1.32.0 Biobase_2.14.0 > > loaded via a namespace (and not attached): > [1] affyio_1.22.0 affyPLM_1.30.0 annotate_1.32.0 > [4] beadarray_2.4.1 BiocInstaller_1.2.1 Biostrings_2.22.0 > [7] Cairo_1.5-0 cluster_1.14.1 DBI_0.2-5 > [10] genefilter_1.36.0 grid_2.14.0 Hmisc_3.9-0 > [13] hwriter_1.3 IRanges_1.12.4 KernSmooth_2.23-7 > [16] lattice_0.20-0 latticeExtra_0.6-19 limma_3.10.0 > [19] preprocessCore_1.16.0 RColorBrewer_1.0-5 RSQLite_0.11.0 > [22] setRNG_2009.11-1 splines_2.14.0 survival_2.36-10 > [25] SVGAnnotation_0.9-0 tools_2.14.0 vsn_3.22.0 > [28] XML_3.6-1 xtable_1.6-0 zlibbioc_1.0.0 > > Again, thank you for help and arrayQualityMetrics. > > Regards. > > Vladimir. > >> Wolfgang Huber<whuber at="" ...=""> writes: >> >> Dear Vladimir >> >> thank you for the precise report. This is peculiar, I just ran your >> example script on a very similar installation (see below), and Figure 3, >> the PCA plot, comes out as intended (i.e. interactive). >> >> Do you get the message "Note: the figure is static - enhancement with >> interactive effects failed..." ? >> >> This is what I tried: >> >> $ uname -a >> Darwin spinoza 11.2.0 Darwin Kernel Version 11.2.0: Tue Aug 9 20:54:00 >> PDT 2011; root:xnu-1699.24.8~1/RELEASE_X86_64 x86_64 >> >> $ pkg-config --modversion cairo libxml-2.0 >> 1.10.2 >> 2.7.8 >> >> > sessionInfo() >> R Under development (unstable) (2011-12-02 r57789) >> Platform: x86_64-apple-darwin11.2.0/x86_64 (64-bit) >> >> locale: >> [1] C >> >> attached base packages: >> [1] stats graphics grDevices utils datasets methods base >> >> other attached packages: >> [1] hgu133acdf_2.9.1 AnnotationDbi_1.17.9 >> [3] arrayQualityMetrics_3.11.0 ALLMLL_1.2.11 >> [5] affy_1.33.2 Biobase_2.15.2 >> [7] BiocGenerics_0.1.3 BiocInstaller_1.3.3 >> [9] fortunes_1.4-2 >> >> loaded via a namespace (and not attached): >> [1] Biostrings_2.23.4 Cairo_1.5-0 DBI_0.2-5 >> [4] Hmisc_3.9-0 IRanges_1.13.13 KernSmooth_2.23-7 >> [7] RColorBrewer_1.0-5 RSQLite_0.11.0 SVGAnnotation_0.9-0 >> [10] XML_3.6-1 affyPLM_1.31.0 affyio_1.23.1 >> [13] annotate_1.33.0 beadarray_2.5.2 cluster_1.14.1 >> [16] genefilter_1.37.0 grid_2.15.0 hwriter_1.3 >> [19] lattice_0.20-0 latticeExtra_0.6-19 limma_3.11.1 >> [22] preprocessCore_1.17.1 setRNG_2009.11-1 splines_2.15.0 >> [25] survival_2.36-10 tools_2.15.0 vsn_3.23.0 >> [28] xtable_1.6-0 zlibbioc_1.1.0 >> >> There haven't been any relevant changes from arrayQualityMetrics_3.10.0 >> to arrayQualityMetrics_3.11.0, so only the potentially relevant >> difference I can see is the locale. Can you try with "C"? >> >> Also, can you make the report that you get available, so I can see what >> the differences in the generated SVG are to the one that I get. This >> could then, potentially, be useful information for the maintainer of the >> SVGAnnotation package (on which arrayQualityMetrics relies) if your >> problem is indeed related to that. >> >> Thanks and best wishes >> Wolfgang >> >> On 12/3/11 2:40 AM, Vladimir Zhurov wrote: >>>> This is the first post, so I am not top-posting. >>> >>> Hello all, >>> >>> I am having a problem with the arrayQualityMetrics interactive >>> SVG graphics. >>> The system is Ubuntu 10.04.3 LTS. Viewing was done in Firefox 8 and >>> Chrome 15. >>> >>> I would greatly appreciate any help with this issue. >>> >>> Below is the information about system, libraries version >>> and sample R session. >>> >>> $ uname -a >>> Linux 2.6.32-36-generic #79-Ubuntu SMP Tue Nov 8 22:29:53 UTC 2011 >>> x86_64 GNU/Linux >>> >>> $ pkg-config --modversion cairo libxml-2.0 >>> 1.10.2 >>> 2.7.8 >>> >>> $ R --vanilla >>> >>> R version 2.14.0 (2011-10-31) >>> Copyright (C) 2011 The R Foundation for Statistical Computing >>> ISBN 3-900051-07-0 >>> Platform: x86_64-pc-linux-gnu (64-bit) >>> >>> R is free software and comes with ABSOLUTELY NO WARRANTY. >>> You are welcome to redistribute it under certain conditions. >>> Type 'license()' or 'licence()' for distribution details. >>> >>> Natural language support but running in an English locale >>> >>> R is a collaborative project with many contributors. >>> Type 'contributors()' for more information and >>> 'citation()' on how to cite R or R packages in publications. >>> >>> Type 'demo()' for some demos, 'help()' for on-line help, or >>> 'help.start()' for an HTML browser interface to help. >>> Type 'q()' to quit R. >>> >>>> library("ALLMLL") >>> Loading required package: affy >>> Loading required package: Biobase >>> >>> Welcome to Bioconductor >>> >>> Vignettes contain introductory material. To view, type >>> 'browseVignettes()'. To cite Bioconductor, see >>> 'citation("Biobase")' and for packages 'citation("pkgname")'. >>> >>>> data("MLL.A") >>>> library("arrayQualityMetrics") >>>> arrayQualityMetrics(MLL.A, >>> + outdir="MLL.A_Report", >>> + force=TRUE, >>> + do.logtransform=TRUE) >>> The directory 'MLL.A_Report' has been created. >>> Loading required package: AnnotationDbi >>> >>> KernSmooth 2.23 loaded >>> Copyright M. P. Wand 1997-2009 >>>> traceback() >>> No traceback available >>>> warnings() >>> NULL >>>> sessionInfo() >>> R version 2.14.0 (2011-10-31) >>> Platform: x86_64-pc-linux-gnu (64-bit) >>> >>> locale: >>> [1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C >>> [3] LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8 >>> [5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8 >>> [7] LC_PAPER=C LC_NAME=C >>> [9] LC_ADDRESS=C LC_TELEPHONE=C >>> [11] LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C >>> >>> attached base packages: >>> [1] stats graphics grDevices utils datasets methods base >>> >>> other attached packages: >>> [1] hgu133acdf_2.9.1 AnnotationDbi_1.16.8 >>> [3] arrayQualityMetrics_3.10.0 ALLMLL_1.2.11 >>> [5] affy_1.32.0 Biobase_2.14.0 >>> >>> loaded via a namespace (and not attached): >>> [1] affyio_1.22.0 affyPLM_1.30.0 annotate_1.32.0 >>> [4] beadarray_2.4.1 BiocInstaller_1.2.1 Biostrings_2.22.0 >>> [7] Cairo_1.5-0 cluster_1.14.1 DBI_0.2-5 >>> [10] genefilter_1.36.0 grid_2.14.0 Hmisc_3.9-0 >>> [13] hwriter_1.3 IRanges_1.12.4 KernSmooth_2.23-7 >>> [16] lattice_0.20-0 latticeExtra_0.6-19 limma_3.10.0 >>> [19] preprocessCore_1.16.0 RColorBrewer_1.0-5 RSQLite_0.11.0 >>> [22] setRNG_2009.11-1 splines_2.14.0 survival_2.36-10 >>> [25] SVGAnnotation_0.9-0 tools_2.14.0 vsn_3.22.0 >>> [28] XML_3.6-1 xtable_1.6-0 zlibbioc_1.0.0 >>> >>> More details that I had to move down here due to "top-posting" error from >>> the >>> web form interface. >>> I apologize for the inconvenience. >>> How is one supposed to post a R session? >>> >>> Only figure 3 is broken, figures 6 and 12 are interactive. >>> I have attempted >>> previously discussed fixes such as updating cairo and libxml. >>> >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at ... >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor >> > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Best wishes Wolfgang Wolfgang Huber EMBL http://www.embl.de/research/units/genome_biology/huber
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