Entering edit mode
karlerhard@berkeley.edu
▴
60
@karlerhardberkeleyedu-4569
Last seen 10.2 years ago
Hello all,
Would anyone be willing to help me figure out a way to convert a
"Genome_intervals_stranded" object into a "Genome_intervals" object
(no
strand information)?
I have gff3 files containing gene annotations and I read these files
into
R using the readGFF3 function, but readGFF3 requires that the input
has a
strand column. I would like to remove that strand information to
compare
antisense reads between two libraries.
Is it as simple as removing the strand column of the
"Genome_intervals_stranded" object in R?
Or perhaps there is a way of reading in a strand-less GFF3 file as a
"Genome_intervals" object that does not use the readGFF3 function?
Thanks for any help, I would greatly appreciate it.
Karl Erhard
Graduate Student
UC Berkeley