convert "Genome_intervals_stranded" to "Genome_intervals"? (girafe)
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@karlerhardberkeleyedu-4569
Last seen 9.7 years ago
Hello all, Would anyone be willing to help me figure out a way to convert a "Genome_intervals_stranded" object into a "Genome_intervals" object (no strand information)? I have gff3 files containing gene annotations and I read these files into R using the readGFF3 function, but readGFF3 requires that the input has a strand column. I would like to remove that strand information to compare antisense reads between two libraries. Is it as simple as removing the strand column of the "Genome_intervals_stranded" object in R? Or perhaps there is a way of reading in a strand-less GFF3 file as a "Genome_intervals" object that does not use the readGFF3 function? Thanks for any help, I would greatly appreciate it. Karl Erhard Graduate Student UC Berkeley
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