Dear list, As I had mentioned in an earlier post the NUSE plots for Affy ST1.0 Gene chips are highly asymmetric and indeed appear to be all positive. Empirically, I have found that four older chips such as mouse 430.2 NUSE boxes which extend over 1.05 are suspect. For an ST1.0 Genes analysis, I have 2 arrays with NUSE plots over 1.05. Is this indicative of a need for rejecting the array? Thanks and best wishes, Rich ------------------------------------------------------------ Richard A. Friedman, PhD Associate Research Scientist, Biomedical Informatics Shared Resource Herbert Irving Comprehensive Cancer Center (HICCC) Lecturer, Department of Biomedical Informatics (DBMI) Educational Coordinator, Center for Computational Biology and Bioinformatics (C2B2)/ National Center for Multiscale Analysis of Genomic Networks (MAGNet) Room 824 Irving Cancer Research Center Columbia University 1130 St. Nicholas Ave New York, NY 10032 (212)851-4765 (voice) friedman at cancercenter.columbia.edu http://cancercenter.columbia.edu/~friedman/ "You can do a minuet to a mazurka if you try hard enough but it will look funny" Judith Friedman, age withheld by request