Dear Byung-Kwan, I could have implemented the function as what you are using. Currently, you will need to read in the data as x = read.table("mydata.gff", sep="\t", header=FALSE), then call GFF2RangedData(x). For detailed information, please type ?BED2RangedData. Thanks! Best regards, Julie On 3/11/12 6:03 AM, "bcho at kaist.ac.kr" <bcho at="" kaist.ac.kr=""> wrote: > Dear Dr. Zhu, > > > I am a very beginner in R and your ChIPpeakAnno package. > > To convert my gff file to RangedData format, I tried to > >> GFF2RangedData(?mydata.gff?, header = FALSE) > > but I got this problem > >> No valid mydata.gff passed in. ..... > > > How can I solve this problem? I am attaching my peak file which is obtained > from NimbleScan. > > Please give my your advise and thanks in advance. > > > > Byung-Kwan >