F p.value.adj missing from write.fit/limma
1
0
Entering edit mode
@pascal-gellert-4249
Last seen 9.7 years ago
Dear Bioconductor members, I am using limma to analyze my microarrays. Therefore I apply lmFit and eBayes on my normalized data. Finally I write the result to a text file by write.fit function. For multiple testing correction I chose adjust='fdr' in the write.fit function. Usually everything works just as it should. However in some cases, the result file does not contain the column "F p.value.adj". The columns with the adjusted p-values ("p.value.adj") and p-value for the F-statistic ("F p.value") are present. Obviously I am doing something wrong, but I cannot figure out what it is since I am using exactly the same code for different data sets and the "F p.value.adj" column does not show up for one data set only. Thanks for your help, Pascal R version 2.10.1 (2009-12-14) limma_3.2.3
limma limma • 1.2k views
ADD COMMENT
0
Entering edit mode
@james-w-macdonald-5106
Last seen 4 days ago
United States
Hi Pascal, On 3/29/2012 9:20 AM, Pascal Gellert wrote: > Dear Bioconductor members, > > I am using limma to analyze my microarrays. Therefore I apply lmFit > and eBayes on my normalized data. Finally I write the result to a text > file by write.fit function. For multiple testing correction I chose > adjust='fdr' in the write.fit function. Usually everything works just > as it should. However in some cases, the result file does not contain > the column "F p.value.adj". The columns with the adjusted p-values > ("p.value.adj") and p-value for the F-statistic ("F p.value") are > present. > Obviously I am doing something wrong, but I cannot figure out what it > is since I am using exactly the same code for different data sets and > the "F p.value.adj" column does not show up for one data set only. > > Thanks for your help, > > Pascal > > > R version 2.10.1 (2009-12-14) > limma_3.2.3 You need to do two things first. One, update to a current version of R. Two, give a small, reproducible code example. Best, Jim > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
ADD COMMENT
0
Entering edit mode
Thanks Jim, you are absolutely right. Pasting the code would have clearly shown that I did something wrong. Meanwhile I figured out myself, that the F p-value gets only corrected with an additional parameter like here: write.fit(fit2, file="file", sep="\t", adjust='fdr', F.adjust='fdr') Pascal On 03/29/2012 03:51 PM, James W. MacDonald wrote: > Hi Pascal, > > On 3/29/2012 9:20 AM, Pascal Gellert wrote: >> Dear Bioconductor members, >> >> I am using limma to analyze my microarrays. Therefore I apply lmFit >> and eBayes on my normalized data. Finally I write the result to a >> text file by write.fit function. For multiple testing correction I >> chose adjust='fdr' in the write.fit function. Usually everything >> works just as it should. However in some cases, the result file does >> not contain the column "F p.value.adj". The columns with the adjusted >> p-values ("p.value.adj") and p-value for the F-statistic ("F >> p.value") are present. >> Obviously I am doing something wrong, but I cannot figure out what it >> is since I am using exactly the same code for different data sets and >> the "F p.value.adj" column does not show up for one data set only. >> >> Thanks for your help, >> >> Pascal >> >> >> R version 2.10.1 (2009-12-14) >> limma_3.2.3 > > You need to do two things first. One, update to a current version of > R. Two, give a small, reproducible code example. > > Best, > > Jim > > >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >
ADD REPLY

Login before adding your answer.

Traffic: 272 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6