F p.value.adj missing from write.fit/limma
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@pascal-gellert-4249
Last seen 10.2 years ago
Dear Bioconductor members, I am using limma to analyze my microarrays. Therefore I apply lmFit and eBayes on my normalized data. Finally I write the result to a text file by write.fit function. For multiple testing correction I chose adjust='fdr' in the write.fit function. Usually everything works just as it should. However in some cases, the result file does not contain the column "F p.value.adj". The columns with the adjusted p-values ("p.value.adj") and p-value for the F-statistic ("F p.value") are present. Obviously I am doing something wrong, but I cannot figure out what it is since I am using exactly the same code for different data sets and the "F p.value.adj" column does not show up for one data set only. Thanks for your help, Pascal R version 2.10.1 (2009-12-14) limma_3.2.3
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@james-w-macdonald-5106
Last seen 13 hours ago
United States
Hi Pascal, On 3/29/2012 9:20 AM, Pascal Gellert wrote: > Dear Bioconductor members, > > I am using limma to analyze my microarrays. Therefore I apply lmFit > and eBayes on my normalized data. Finally I write the result to a text > file by write.fit function. For multiple testing correction I chose > adjust='fdr' in the write.fit function. Usually everything works just > as it should. However in some cases, the result file does not contain > the column "F p.value.adj". The columns with the adjusted p-values > ("p.value.adj") and p-value for the F-statistic ("F p.value") are > present. > Obviously I am doing something wrong, but I cannot figure out what it > is since I am using exactly the same code for different data sets and > the "F p.value.adj" column does not show up for one data set only. > > Thanks for your help, > > Pascal > > > R version 2.10.1 (2009-12-14) > limma_3.2.3 You need to do two things first. One, update to a current version of R. Two, give a small, reproducible code example. Best, Jim > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099
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Thanks Jim, you are absolutely right. Pasting the code would have clearly shown that I did something wrong. Meanwhile I figured out myself, that the F p-value gets only corrected with an additional parameter like here: write.fit(fit2, file="file", sep="\t", adjust='fdr', F.adjust='fdr') Pascal On 03/29/2012 03:51 PM, James W. MacDonald wrote: > Hi Pascal, > > On 3/29/2012 9:20 AM, Pascal Gellert wrote: >> Dear Bioconductor members, >> >> I am using limma to analyze my microarrays. Therefore I apply lmFit >> and eBayes on my normalized data. Finally I write the result to a >> text file by write.fit function. For multiple testing correction I >> chose adjust='fdr' in the write.fit function. Usually everything >> works just as it should. However in some cases, the result file does >> not contain the column "F p.value.adj". The columns with the adjusted >> p-values ("p.value.adj") and p-value for the F-statistic ("F >> p.value") are present. >> Obviously I am doing something wrong, but I cannot figure out what it >> is since I am using exactly the same code for different data sets and >> the "F p.value.adj" column does not show up for one data set only. >> >> Thanks for your help, >> >> Pascal >> >> >> R version 2.10.1 (2009-12-14) >> limma_3.2.3 > > You need to do two things first. One, update to a current version of > R. Two, give a small, reproducible code example. > > Best, > > Jim > > >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >
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