Question

Selected probes from a given probeset

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Ramiro Rodríguez ▴ 30

@ramiro-rodriguez-5244

Last seen 10.2 years ago

Dear list, I?m Bioconductor for Arabidosis AtH1 microarray analysis. I was wondering if there is a way to get expression values for a given gene (probeset), but using only a subset of the probes from the probeset. Thanks to all in advance, Ramiro. -- Dr. Ramiro E. Rodriguez Investigador Asistente de CONICET Instituto de Biolog?a Molecular y Celular de Rosario (IBR) - CONICET Suipacha 531 - S2002LRK - Rosario, Santa Fe ARGENTINA TEL: 54-341-4351235 (Ext. 147) Fax: 54-341-4390465 E-mail: rrodriguez at ibr.gov.ar

Microarray SANTA Microarray SANTA • 1.3k views

ADD COMMENT • link 12.6 years ago Ramiro Rodríguez ▴ 30

score 0 · Answer 1 · 2012-04-23

Hi Ramiro, On 4/20/2012 3:00 PM, Ramiro Rodr?guez wrote: > Dear list, > I?m Bioconductor for Arabidosis AtH1 microarray analysis. > I was wondering if there is a way to get expression values for a given > gene (probeset), but using only a subset of the probes from the probeset. There is some code that was originally written by Ariel Chernomoretz and further modified by Jenny Drnevich that has been used by various people over the years. I am not one of them (the users), so don't know the ins and outs of using the script, but you can find it here: https://stat.ethz.ch/pipermail/bioconductor/2006-September/014242.html Best, Jim > Thanks to all in advance, > Ramiro. > -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099

score 0 · Answer 2 · 2012-04-25

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Ramiro Rodríguez ▴ 30

@ramiro-rodriguez-5244

Last seen 10.2 years ago

Dear list, I?m using Bioconductor for Affymetrix AtH1 microarray analysis. I was wondering if there is a way to get expression values for a given gene (probeset), but using only a subset of the probes from the probeset. Thanks to all in advance, Ramiro. -- Dr. Ramiro E. Rodriguez Investigador Asistente de CONICET Instituto de Biolog?a Molecular y Celular de Rosario (IBR) - CONICET Suipacha 531 - S2002LRK - Rosario, Santa Fe ARGENTINA TEL: 54-341-4351235 (Ext. 147) Fax: 54-341-4390465 E-mail: rrodriguez at ibr.gov.ar

ADD COMMENT • link 12.6 years ago Ramiro Rodríguez ▴ 30

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Dear list, I?m using Bioconductor for Affymetrix AtH1 microarray analysis. I was wondering if there is a way to get expression values for a given gene (probeset), but using only a subset of the probes from the probeset. Thanks to all in advance, Ramiro. -- Dr. Ramiro E. Rodriguez Investigador Asistente de CONICET Instituto de Biolog?a Molecular y Celular de Rosario (IBR) - CONICET Suipacha 531 - S2002LRK - Rosario, Santa Fe ARGENTINA TEL: 54-341-4351235 (Ext. 147) Fax: 54-341-4390465 E-mail: rrodriguez at ibr.gov.ar

ADD REPLY • link 12.6 years ago Ramiro Rodriguez ▴ 10

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Hi Ramiro, Two things. First, repeatedly asking the same question will not gain you any friends. If nobody answers perhaps you could take it upon yourself to figure out how to do this. Second, I answered this question five days ago when you first asked. And you in fact asked this question a second time by responding to the email you got as my response, without even acknowledging that you had already got an answer. This leads me to wonder what was wrong with the answer you already have? Best, Jim On 4/25/2012 11:13 AM, Ramiro Rodriguez wrote: > > Dear list, > I?m using Bioconductor for Affymetrix AtH1 microarray analysis. > I was wondering if there is a way to get expression values for a given > gene (probeset), but using only a subset of the probes from the probeset. > Thanks to all in advance, > Ramiro. > -- James W. MacDonald, M.S. Biostatistician University of Washington Environmental and Occupational Health Sciences 4225 Roosevelt Way NE, # 100 Seattle WA 98105-6099

ADD REPLY • link 12.6 years ago James W. MacDonald 67k

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Hi Jim, Sorry, but I just missed your response on April 23 to my question, which now I could found, and I of course acknowledge very much. Best, Ramiro. El 26/04/2012 08:04 a.m., James W. MacDonald escribi?: > Hi Ramiro, > > Two things. > > First, repeatedly asking the same question will not gain you any > friends. If nobody answers perhaps you could take it upon yourself to > figure out how to do this. > > Second, I answered this question five days ago when you first asked. > And you in fact asked this question a second time by responding to the > email you got as my response, without even acknowledging that you had > already got an answer. This leads me to wonder what was wrong with the > answer you already have? > > Best, > > Jim > > > > On 4/25/2012 11:13 AM, Ramiro Rodriguez wrote: >> >> Dear list, >> I?m using Bioconductor for Affymetrix AtH1 microarray analysis. >> I was wondering if there is a way to get expression values for a given >> gene (probeset), but using only a subset of the probes from the >> probeset. >> Thanks to all in advance, >> Ramiro. >> > -- Dr. Ramiro E. Rodriguez Investigador Asistente de CONICET Instituto de Biolog?a Molecular y Celular de Rosario (IBR) - CONICET Suipacha 531 - S2002LRK - Rosario, Santa Fe ARGENTINA TEL: 54-341-4351235 (Ext. 147) Fax: 54-341-4390465 E-mail: rrodriguez at ibr.gov.ar

ADD REPLY • link 12.6 years ago Ramiro Rodríguez ▴ 30