hi, I have 2 RT-PCR microfluidics cards but done on different days: - card A has 51 samples and 48 genes - card B has 160 samples and 96 genes They have in common 7 samples and 41 genes. In both cards each gene is measured in duplicate. I might use HTqPCR functions for instance to analyze them. But any hint about how can I merge the two cards in a single dataset in a way to moderate/eliminate the putative day and card bias? regards, Alex ------------------------------------------------ Alessandro Brozzi,* *PhD Department of Mathematics and Informatics University of Perugia via Vanvitelli, 1 06123 Perugia, ITALY office n. 507 tel: +39 075 585 5039 mail: alessandro.brozzi[@]dmi.unipg.it [[alternative HTML version deleted]]