Qspline (again)
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Laurent Gautier ★ 2.3k
@laurent-gautier-29
Last seen 9.7 years ago
On Wed, Oct 30, 2002 at 04:15:52PM -0500, Paul Boutros wrote: > Hi again, > > > > > > The error message I get is: > > > > > Error in rep(data, t1) : invalid number of copies in "rep" > > > > > > > > > > > > > Try 'traceback()' just after the error message is fired. It may give > > > > us a hint about where this happened. > > > > > > I get: > > > 3: rep(data, t1) > > > 2: array(y.offset, (k - 1)) > > > 1: normalize.qspline(data2) > > > > > > Does this help at all? > > > It does. I had a hard time with the 'k' thing (and no answer from the > > corresponding author about my questions/remarks/suggestions). I suspect > > that for some reason you do not have the check added. Does the code > > for the function look like follows ? > > ------------------- > > if (y.offset <= min.offset) { > > y.offset <- min.offset; > > k <- round(py.inds[1]/min.offset) > > } > > > > ## here is the part I suspect missing ## > > if (k < 1) { > > warning("qspline cannot be performed (insufficient number of > arrays)") > > return(x) > > } > > ## ---- ## > > > > y.offset <- c(0, array(y.offset, (k-1))) > > y.order <- order(target) > > ------------------- > > This is exactly how my code looks -- the check on array number is not > present. > However, I should note that I am doing this analysis with three arrays. > The code I use looks like this: > > > data1 <- read.table("c:\\docume~1\\paul\\dev\\testvals.txt"); > > data2 <- data.matrix(data1); > > data2; > qry1.S635 qry1.S532 qry2.S635 qry2.S532 qry3.S635 qry3.S532 > H3001A10 613.0 602.5 483.0 633.0 61.0 75.0 > H3001A12 1208.5 1019.0 1209.0 1187.0 91.0 153.5 > H3001B01 337.0 353.0 619.0 529.5 132.5 153.5 > H3001C02 495.0 497.5 64.5 117.5 10.5 59.0 > H3001C03 199.5 152.5 301.0 222.0 63.5 95.5 > H3001C04 1855.5 1447.0 1969.0 1721.0 286.0 349.0 > H3001C07 4765.5 3643.0 4889.0 4401.5 1064.0 1148.0 > H3001C09 720.5 894.5 347.0 602.0 188.0 326.5 > H3001C11 630.0 536.0 814.5 899.5 20.0 48.5 > > c <- normalize.qspline(data2); > Error in rep(data, t1) : invalid number of copies in "rep" > > c <- normalize.qspline(data1); > Error in rep(data, t1) : invalid number of copies in "rep" > > I tried adding the code you indicated as missing into the affy.R file. > I now get: > > qry1.S635 qry1.S532 qry2.S635 qry2.S532 qry3.S635 qry3.S532 > H3001A10 613.0 602.5 483.0 633.0 61.0 75.0 > H3001A12 1208.5 1019.0 1209.0 1187.0 91.0 153.5 > H3001B01 337.0 353.0 619.0 529.5 132.5 153.5 > H3001C02 495.0 497.5 64.5 117.5 10.5 59.0 > H3001C03 199.5 152.5 301.0 222.0 63.5 95.5 > H3001C04 1855.5 1447.0 1969.0 1721.0 286.0 349.0 > H3001C07 4765.5 3643.0 4889.0 4401.5 1064.0 1148.0 > H3001C09 720.5 894.5 347.0 602.0 188.0 326.5 > H3001C11 630.0 536.0 814.5 899.5 20.0 48.5 > > Warning message: > qspline cannot be performed (insufficient number of arrays) in: > normalize.qspline(data2) > > This indicates to me that my input data is in an inappropriate format > somehow? No. The format seems to be appropriate. > Any ideas where I go from here? > I do. As mentioned earlier the variable 'k' is estimated in the code through an iterative procedure (which has the annoying property to give a value that do not make sense in some cases). I contacted the corresponding author about that but get no answer. I inserted the error message "insufficient blahblablah" since I mainly observed that when applied with to a small number of arrays. One way to get something done, waiting for the day I will have time to go in details into that, would be to set a default value for k when the iterative things do not behave nicely. Try to replace what you inserted with : ## ----------- ## if (k < 1) { warning("'k' things did not work. Set to default value") k <- 5 } ## ---- ## Hopin' it helps, Laurent
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Paul Boutros ▴ 200
@paul-boutros-98
Last seen 9.7 years ago
Hi again, This works great now. Thanks for your help! Paul On Tue, 5 Nov 2002, Laurent Gautier wrote: > On Wed, Oct 30, 2002 at 04:15:52PM -0500, Paul Boutros wrote: > > Hi again, > > > > > > > > The error message I get is: > > > > > > Error in rep(data, t1) : invalid number of copies in "rep" > > > > > > > > > > > > > > > > Try 'traceback()' just after the error message is fired. It may give > > > > > us a hint about where this happened. > > > > > > > > I get: > > > > 3: rep(data, t1) > > > > 2: array(y.offset, (k - 1)) > > > > 1: normalize.qspline(data2) > > > > > > > > Does this help at all? > > > > > It does. I had a hard time with the 'k' thing (and no answer from the > > > corresponding author about my questions/remarks/suggestions). I suspect > > > that for some reason you do not have the check added. Does the code > > > for the function look like follows ? > > > ------------------- > > > if (y.offset <= min.offset) { > > > y.offset <- min.offset; > > > k <- round(py.inds[1]/min.offset) > > > } > > > > > > ## here is the part I suspect missing ## > > > if (k < 1) { > > > warning("qspline cannot be performed (insufficient number of > > arrays)") > > > return(x) > > > } > > > ## ---- ## > > > > > > y.offset <- c(0, array(y.offset, (k-1))) > > > y.order <- order(target) > > > ------------------- > > > > This is exactly how my code looks -- the check on array number is not > > present. > > However, I should note that I am doing this analysis with three arrays. > > The code I use looks like this: > > > > > data1 <- read.table("c:\\docume~1\\paul\\dev\\testvals.txt"); > > > data2 <- data.matrix(data1); > > > data2; > > qry1.S635 qry1.S532 qry2.S635 qry2.S532 qry3.S635 qry3.S532 > > H3001A10 613.0 602.5 483.0 633.0 61.0 75.0 > > H3001A12 1208.5 1019.0 1209.0 1187.0 91.0 153.5 > > H3001B01 337.0 353.0 619.0 529.5 132.5 153.5 > > H3001C02 495.0 497.5 64.5 117.5 10.5 59.0 > > H3001C03 199.5 152.5 301.0 222.0 63.5 95.5 > > H3001C04 1855.5 1447.0 1969.0 1721.0 286.0 349.0 > > H3001C07 4765.5 3643.0 4889.0 4401.5 1064.0 1148.0 > > H3001C09 720.5 894.5 347.0 602.0 188.0 326.5 > > H3001C11 630.0 536.0 814.5 899.5 20.0 48.5 > > > c <- normalize.qspline(data2); > > Error in rep(data, t1) : invalid number of copies in "rep" > > > c <- normalize.qspline(data1); > > Error in rep(data, t1) : invalid number of copies in "rep" > > > > I tried adding the code you indicated as missing into the affy.R file. > > I now get: > > > > qry1.S635 qry1.S532 qry2.S635 qry2.S532 qry3.S635 qry3.S532 > > H3001A10 613.0 602.5 483.0 633.0 61.0 75.0 > > H3001A12 1208.5 1019.0 1209.0 1187.0 91.0 153.5 > > H3001B01 337.0 353.0 619.0 529.5 132.5 153.5 > > H3001C02 495.0 497.5 64.5 117.5 10.5 59.0 > > H3001C03 199.5 152.5 301.0 222.0 63.5 95.5 > > H3001C04 1855.5 1447.0 1969.0 1721.0 286.0 349.0 > > H3001C07 4765.5 3643.0 4889.0 4401.5 1064.0 1148.0 > > H3001C09 720.5 894.5 347.0 602.0 188.0 326.5 > > H3001C11 630.0 536.0 814.5 899.5 20.0 48.5 > > > > Warning message: > > qspline cannot be performed (insufficient number of arrays) in: > > normalize.qspline(data2) > > > > This indicates to me that my input data is in an inappropriate format > > somehow? > > No. The format seems to be appropriate. > > > > Any ideas where I go from here? > > > > I do. As mentioned earlier the variable 'k' is estimated in the code > through an iterative procedure (which has the annoying property to > give a value that do not make sense in some cases). > I contacted the corresponding author about that but get no answer. > I inserted the error message "insufficient blahblablah" since I > mainly observed that when applied with to a small number of arrays. > One way to get something done, waiting for the day I will have time to > go in details into that, would be to set a default value for k when the > iterative things do not behave nicely. > > Try to replace what you inserted with : > > ## ----------- ## > if (k < 1) { > warning("'k' things did not work. Set to default value") > k <- 5 > } > ## ---- ## > > > Hopin' it helps, > > > > > Laurent > > > >
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A note to anybody following this discussion. I found that, another solution to Laurents' idea of setting k <- 5 as a default is altering the samples parameter. Depending on the dataset size, a different number of samples may be optimal. For instance: ~200 data points use samples = 0.33 ~2000 data points use samples = 0.05 ~10000 data points use samples = 0.02 So that implies that perhaps the default sampling rate was just too large for the data set I was using. Hope this helps! Paul On Tue, 5 Nov 2002, Paul Boutros wrote: > Hi again, > > This works great now. Thanks for your help! > Paul > > On Tue, 5 Nov 2002, Laurent Gautier wrote: > > > On Wed, Oct 30, 2002 at 04:15:52PM -0500, Paul Boutros wrote: > > > Hi again, > > > > > > > > > > The error message I get is: > > > > > > > Error in rep(data, t1) : invalid number of copies in "rep" > > > > > > > > > > > > > > > > > > > Try 'traceback()' just after the error message is fired. It may give > > > > > > us a hint about where this happened. > > > > > > > > > > I get: > > > > > 3: rep(data, t1) > > > > > 2: array(y.offset, (k - 1)) > > > > > 1: normalize.qspline(data2) > > > > > > > > > > Does this help at all? > > > > > > > It does. I had a hard time with the 'k' thing (and no answer from the > > > > corresponding author about my questions/remarks/suggestions). I suspect > > > > that for some reason you do not have the check added. Does the code > > > > for the function look like follows ? > > > > ------------------- > > > > if (y.offset <= min.offset) { > > > > y.offset <- min.offset; > > > > k <- round(py.inds[1]/min.offset) > > > > } > > > > > > > > ## here is the part I suspect missing ## > > > > if (k < 1) { > > > > warning("qspline cannot be performed (insufficient number of > > > arrays)") > > > > return(x) > > > > } > > > > ## ---- ## > > > > > > > > y.offset <- c(0, array(y.offset, (k-1))) > > > > y.order <- order(target) > > > > ------------------- > > > > > > This is exactly how my code looks -- the check on array number is not > > > present. > > > However, I should note that I am doing this analysis with three arrays. > > > The code I use looks like this: > > > > > > > data1 <- read.table("c:\\docume~1\\paul\\dev\\testvals.txt"); > > > > data2 <- data.matrix(data1); > > > > data2; > > > qry1.S635 qry1.S532 qry2.S635 qry2.S532 qry3.S635 qry3.S532 > > > H3001A10 613.0 602.5 483.0 633.0 61.0 75.0 > > > H3001A12 1208.5 1019.0 1209.0 1187.0 91.0 153.5 > > > H3001B01 337.0 353.0 619.0 529.5 132.5 153.5 > > > H3001C02 495.0 497.5 64.5 117.5 10.5 59.0 > > > H3001C03 199.5 152.5 301.0 222.0 63.5 95.5 > > > H3001C04 1855.5 1447.0 1969.0 1721.0 286.0 349.0 > > > H3001C07 4765.5 3643.0 4889.0 4401.5 1064.0 1148.0 > > > H3001C09 720.5 894.5 347.0 602.0 188.0 326.5 > > > H3001C11 630.0 536.0 814.5 899.5 20.0 48.5 > > > > c <- normalize.qspline(data2); > > > Error in rep(data, t1) : invalid number of copies in "rep" > > > > c <- normalize.qspline(data1); > > > Error in rep(data, t1) : invalid number of copies in "rep" > > > > > > I tried adding the code you indicated as missing into the affy.R file. > > > I now get: > > > > > > qry1.S635 qry1.S532 qry2.S635 qry2.S532 qry3.S635 qry3.S532 > > > H3001A10 613.0 602.5 483.0 633.0 61.0 75.0 > > > H3001A12 1208.5 1019.0 1209.0 1187.0 91.0 153.5 > > > H3001B01 337.0 353.0 619.0 529.5 132.5 153.5 > > > H3001C02 495.0 497.5 64.5 117.5 10.5 59.0 > > > H3001C03 199.5 152.5 301.0 222.0 63.5 95.5 > > > H3001C04 1855.5 1447.0 1969.0 1721.0 286.0 349.0 > > > H3001C07 4765.5 3643.0 4889.0 4401.5 1064.0 1148.0 > > > H3001C09 720.5 894.5 347.0 602.0 188.0 326.5 > > > H3001C11 630.0 536.0 814.5 899.5 20.0 48.5 > > > > > > Warning message: > > > qspline cannot be performed (insufficient number of arrays) in: > > > normalize.qspline(data2) > > > > > > This indicates to me that my input data is in an inappropriate format > > > somehow? > > > > No. The format seems to be appropriate. > > > > > > > Any ideas where I go from here? > > > > > > > I do. As mentioned earlier the variable 'k' is estimated in the code > > through an iterative procedure (which has the annoying property to > > give a value that do not make sense in some cases). > > I contacted the corresponding author about that but get no answer. > > I inserted the error message "insufficient blahblablah" since I > > mainly observed that when applied with to a small number of arrays. > > One way to get something done, waiting for the day I will have time to > > go in details into that, would be to set a default value for k when the > > iterative things do not behave nicely. > > > > Try to replace what you inserted with : > > > > ## ----------- ## > > if (k < 1) { > > warning("'k' things did not work. Set to default value") > > k <- 5 > > } > > ## ---- ## > > > > > > Hopin' it helps, > > > > > > > > > > Laurent > > > > > > > > > >
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One last thing about that. Most likely I will not have time to go through the algorithm before the release. I modified the code in the devel version so that 'k' takes the value of the parameter 'fit.iter' in case of non-sense. With Paul's agreement, I will also include his tips in the man page. L. On Thu, Nov 07, 2002 at 04:45:35PM -0500, Paul Boutros wrote: > A note to anybody following this discussion. I found that, another > solution to Laurents' idea of setting k <- 5 as a default is altering the > samples parameter. Depending on the dataset size, a different number of > samples may be optimal. > > For instance: > ~200 data points use samples = 0.33 > ~2000 data points use samples = 0.05 > ~10000 data points use samples = 0.02 > > So that implies that perhaps the default sampling rate was just too large > for the data set I was using. > > Hope this helps! > Paul
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